猪流感病毒H1、H3、N1、N2亚型分型 RT-PCR方法的建立

Abstract

Abstract: According to the genomic sequence of H1N1,H3N2 subtype swine influenza virus (SIV) published in GenBank, the specific primers were designed according to the conserved region of H1,H3 HA,N1,N2 NA and M genes of swine influenza virus (SIV) H1N1,H3N2 subtype. RT-PCR assay was developed for detection of swine influenza H1,H3,N1,N2 subtype. The results showed that the RT-PCR was capable of detecting 10⁴ EID₅₀ of M and 10⁴ EID₅₀ of HA or NA. The results were negative for the detection of porcine reproductive and respiratory syndrome virus,CSFV and other subtypes except the corresponding subtype. The coincidence rates between RT-PCR and virus isolation were 100%. This assay provides a rapid,sensitive,and cost-effective laboratory diagnosis for detecting and subtyping of SIV in pigs.

Key words: swine influenza virus; H1 subtype; H3 subtype; N1 subtype; N2 subtype; RT-PCR

CLC Number:

S852.659.5

QI Hai-tao;KONG Wei-li;ZHANG Gui-hong. Development of RT-PCR Assay for Rapid Detection and Subtyping of Swine Influenza H1,H3,N1,N2 Viruses[J]. China Animal Husbandry & Veterinary Medicine, 2012, 39(3): 187-191.

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Development of RT-PCR Assay for Rapid Detection and Subtyping of Swine Influenza H1,H3,N1,N2 Viruses

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