China Animal Husbandry & Veterinary Medicine ›› 2012, Vol. 39 ›› Issue (12): 212-215.

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Determination of Genhuang Dispersible Tablets by RP-HPLC and the Study of the Stability

XIN Rui-hua1, LUO Yong-jiang1, ZHENG Ji-fang1, LI Wei2, WANG Gui-bo1, LUO Chao-ying1, LI Jin-yu1, XIE Jia-sheng1   

  1. 1. Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS, Lanzhou 730050, China;
    2. Lanzhou Center for Disease Control and Prevention, Lanzhou 730030, China
  • Received:2012-05-28 Online:2012-12-20 Published:2012-12-19

Abstract: The study aimed to establish a method for determining Baicalin in Genhuang dispersible tablets and inspection for the preparation of stability. RP-HPLC method was adopted and the C18 column(150 mm×4.6 mm,10 μm)was used. The mobile phase was methanol-water-phosphoric acid(47∶53∶0.2%), with the flow rate of 1 mL/min. The detection wavelength was at 280 nm and the column temperature was 30 ℃. Under the high-light exposure experiment, accelerating experiment and room temperature storage observation, the results showed that the product was basically stable. The calibration curve of Baicalin was linear in the range of 1.018 to 50.90 μg/mL, r=0.9999(n=6), the average recovery rate was 97.27%, RSD=0.75% (n=9). The method was simple, sensitive and accurate with good repeatability, which can be used for the quality control of Genhuang dispersible tablets, and the preparation of good stability.

Key words: RP-HPLC; Genhuang dispersible tablets; Baicalin; stability

CLC Number: