Abstract: The assay was aimed to investigate the law of trace elements (Cu, Fe, Mn, Zn) and interleukin (IL-1, IL-6) contents in serum using Cyst-regulating powder to treat infertility caused by ovarian disease of dairy cattle.Flame atomic absorption spectrometry and enzyme-linked immunization absorb assay were used to detect levels of the trace elements and interleukin contents in serum from the experimental and normal group before and after treatment.The results were showed as following.①After treatment, the effective rate of follicular cyst was 83.33%, the effective rate of delayed ovulation was 90.91%.②In follicular cyst body, Mn, Zn contents were significantly lower than the normal group in pre-treatment (P<0.05), Fe content was significantly higher than the normal group(P<0.05), after treatment, their content levels were closed to the normal group, and the difference was not significant(P>0.05), the Cu, IL-1, IL-6 contents were closed to the normal group before and after treatment. ③In delayed ovulation, Fe content was signifcantly higher than the normal group(P<0.05),Mn content was extremly significantly lower than the normal group in pre-treatment(P<0.01), Zn content was signifcantly higher than the normal group (P<0.05), after treatment, the contents was not gradually significant(P>0.05),but Cu, IL-1, IL-6 content levels were not significantly compared with the normal group before and after treatment(P>0.05). In conclusion, the effect of trace elements and interleukin in serum can’t be ignored by using Cyst-regulating powder to treat infertility caused by ovarian disease of dairy cattle.

Key words: Cyst-regulating powder; dairy cattle; follicular cyst; delayed ovulation; trace elements; interleukin