绵羊肝片吸虫谷胱甘肽硫转移酶基因的克隆与序列分析

›› 2012, Vol. 39 ›› Issue (1): 64-67.

• 生物技术 • Previous Articles Next Articles

Cloning and Sequence Analysing of GST Gene of Fasciola hepatica Isolated from Sheep

RAN Xu-hua^1,2, WEN Xiao-bo^1,2, WANG Chun-ren², LIU Di¹, SUN Zhong-wu¹, LI Xiao-juan², WANG Mi²

1. Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme,Northeast Forestry University Postdoctoral Programme,Harbin 150030,China;2. College of Animal Science and Technology, Heilongjiang Bayi Agricultural University,Daqing 163319,China

Received:2011-05-16 Revised:1900-01-01 Online:2012-01-20 Published:2012-01-20

Abstract

Abstract: The glutathione S-transferase gene of Fasciola hepatica was amplified and its homology was analyzed. According to the published genomic sequence of Fh GST in GenBank, a pair of primers was designed and the open reading frame of GST gene of Fasciola hepatica was amplified by RT-PCR. The homology of GST gene was analyzed by molecular biological soft. The PCR analysis results showed that the Fh GST gene was 682 bp, and encoded 218 amino acid. It indicated that the Fh GST gene was highly homologous with Fasciola hepatica isolated from Australia, Fasciola gigantica and Paragonimus westermani. Because of the high homology of the GST gene of different parasite, it could not be used in diagnosis assay with Fh GST protein, but could be used in gene vaccine.The clone of Fh GST gene had advantages to investigate the function and effect of GST protein.

Key words: Fasciola hepatica; GST gene; sequence analysis

CLC Number:

RAN Xu-hua;WEN Xiao-bo;WANG Chun-ren;LIU Di;SUN Zhong-wu;LI Xiao-juan;WANG Mi. Cloning and Sequence Analysing of GST Gene of Fasciola hepatica Isolated from Sheep[J]. , 2012, 39(1): 64-67.

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Cloning and Sequence Analysing of GST Gene of Fasciola hepatica Isolated from Sheep

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