动物饲料中沙门氏菌环介导等温扩增(LAMP)快速诊断方法的建立

Abstract

Abstract: According to the highly conserved fimY gene of Salmonella, reported in the GenBank, by primer explorer V4 softward, one set primer including four primers ,were designed and the method for detecting the Salmonella with LAMP was developed after optimizing the reaction conditions. The results showed that the detection limit of the LAMP assay was 6.2 CFU/mL which was 100-fold higher than that of PCR. The test results of 24 samples by LAMP method was fully consistent with these by the national standard method. The results showed the LAMP method is more reliable and convenient for rapid, sensitive and specific diagnosis of Salmonella.

Key words: Salmonella; animal feed; LAMP; rapid detection

CLC Number:

S852.61

SUN Yuan-yuan;ZHAO Peng. Development of a Loop-Mediated Isothermal Amplication Method for Rapid Detection of Salmonella in Animal Food[J]. , 2012, 39(1): 32-36.

[1]	LI Lei, XIAO Shuang, ZHEN Sihui, WANG Yu, WU Zhouhui, WANG Zhiwen, DU Heng, LU Lin, WANG Zhen. Development and Application of Nucleic Acid Aptamer-based Rapid Test Strips for the Detection of Staphylococcus aureus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(11): 4577-4588.
[2]	FAN Lixia, YUAN Xuexia, LI Yuanyang, WU Yuanjuan, ZHAO Shancang, ZHANG Bingchun, WANG Wenbo. LAMP Detection and Drug Resistance Analysis to Staphylococcus aureus and Escherichia coli in Livestock and Poultry Manure [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 2325-2335.
[3]	XIE Jing, YU Jifeng, ZHOU Long, CAO Ye, LIN Yi, LI Xingyu, XIAO Lu, YE Yonggang, PAN Meng, KANG Runmin. Establishment and Application of Visual LAMP Detection Method for Infectious Laryngotracheitis Virus [J]. , 2019, 46(2): 565-572.
[4]	XU Yunming, WEI Libin, ZHOU Yanyang, LI Yehui, SUN Zhiyuan, ZHONG Siyuan, CHEN Aining, GUO Lijuan, LIU Zengshan, REN Honglin. Establishment of Loop-mediated Isothermal Amplification (LAMP) Method for Klebsiella pneumoniae [J]. , 2018, 45(11): 3003-3010.
[5]	LIU Wenjun, HUANG Yunmao, YANG Youtian, XU Danning, CAO Nan, ZHOU Derong, TIAN Yunbo. Establishment of RT-LAMP Visual Detection Method for Goose Newcastle Disease Virus [J]. , 2018, 45(1): 22-31.
[6]	LI Dan-ni, HUANG Hua, ZHANG Jing, WANG Xue-sheng, GU Yi-wen. Preparation and Properties Analysis of Three β₂-agonists Fluorescent Immunochromatographic Strip [J]. , 2017, 44(8): 2437-2442.
[7]	CHEN Xi-wen, WANG Qian, YIN Miao, LI Lian, LUO Wen-tao, YANG Feng, GUO Ai-wei, ZHOU Jie-long, WANG Xiong-qing. Establishment of RT-LAMP for Rapid Detection of Porcine Reproductive and Respiratory Syndrome Virus [J]. , 2017, 44(6): 1630-1636.
[8]	HAN Xiao-ya, CHEN Dong-mei, WANG Yu-lian, TAO Yan-fei, DONG Guo-liang, PENG DA-peng, YUAN Zong-hui. Research Progress on Antibodies Rapid Detection Technology for Veterinary Synthetic Antibacterial Agents [J]. , 2017, 44(6): 1869-1876.
[9]	LUO Ya-kun, LIANG Lin, WANG Jing, LIU Cun, LIU Qi, LIU Chang, LIN Wen-cheng, CUI Shang-jin. Establishment and Application of Loop-mediated Isothermal Amplification for Rapid Detection of Porcine Epidemic Diarrhea Virus [J]. , 2017, 44(2): 344-349.
[10]	LIU Li-bing, WANG Jian-chang, JING Mei, WANG Jin-feng, YUAN Wan-zhe. Development of the Recombinase Polymerase Amplification Assay for Rapid Detection of Porcine Parvovirus [J]. , 2017, 44(11): 3320-3326.
[11]	WU Zhi, XIA Wen-long, CAI Shu-dong, GUO Chang-ming, YUAN Wei-feng, WANG Yong-juan. Establishment and Application of Visual RT-LAMP for Rapid Detection of Duck Tembusu Virus [J]. , 2017, 44(11): 3334-3339.
[12]	BAI Yu, ZHANG Jing, HU Jing-yan, XU Yong-peng, WANG Shu-ming. Study on Colloidal Gold Immunochromatographic Assay for Rapid Detection of Estradiol in Milk [J]. , 2017, 44(11): 3351-3357.
[13]	LI Jian-you, LI Jia-wei, WANG Chao, GUO Li, HE Hong-bin. Establishment and Preliminary Application of Nano-PCR and LAMP Methods for Bovine Parainfluenza Type-3 Virus [J]. , 2017, 44(10): 2837-2844.
[14]	NI Teng-teng, PENG Da-peng, XIE Shu-yu, CHEN Dong-mei, WANG Yu-lian, PAN Yuan-hu, TAO Yan-fei, YUAN Zong-hui. Research Progress on Rapid Determination Technology of Avermectins in Feeds and Animal Products [J]. , 2016, 43(9): 2344-2351.
[15]	YANG Min, WANG Hui-dang, YU Lu, WU Chang-xin, Zhang Hui, CAO Xu-dong, CHEN Chuang-fu. Study on Optimization of the Conditions of Detecting Mycobacterium tuberculosis in Milk by Technique of Loop-mediated Isothermal Amplification [J]. , 2016, 43(7): 1688-1693.

Development of a Loop-Mediated Isothermal Amplication Method for Rapid Detection of Salmonella in Animal Food

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments