Abstract: According to the highly conserved fimY gene of Salmonella, reported in the GenBank, by primer explorer V4 softward, one set primer including four primers ,were designed and the method for detecting the Salmonella with LAMP was developed after optimizing the reaction conditions. The results showed that the detection limit of the LAMP assay was 6.2 CFU/mL which was 100-fold higher than that of PCR. The test results of 24 samples by LAMP method was fully consistent with these by the national standard method. The results showed the LAMP method is more reliable and convenient for rapid, sensitive and specific diagnosis of Salmonella.

Key words: Salmonella; animal feed; LAMP; rapid detection