Abstract: Bovine ZnF312 gene was isolated using RT-PCR and sequencing, the results showed that its cDNA sequence was 1812 bp, including 63 bp 5'-UTR, 1377 bp CDS and 372 bp 3'-UTR. The results of bio-informatics analysis for the structure and function of ZnF312 gene and its coded protein showed that ZnF312 gene was located on chromosome 22 and coded 458 amino acid. The molecular weight and isoelectric point of ZnF312 protein were 48.82 ku and 9.419, respectively. ZnF312 protein contained six ZnF_C2H2 domains, located on many cell organs, such as cell nucleus (95.7% properties) and mitochondria (4.3% properties). According the results in this study and the function of ZnF_C2H2 domain, it was inferred that ZnF312 may be very important for growth, disease resistance and susceptibility by identify the corresponding target genes.

Key words: cattle; ZnF312 gene; gene cloning; bio-informatics