Abstract: DNA of three types of avian Mycoplasma (Mycoplasma gallisepticum,MG; Mycoplasma synoviae, MS and Mycoplasma meleagridis, MM) were extracted and amplified through single polymerase chain reaction (PCR) and multiplex PCR with three sets of specific oligonucleotide primers. There were consistent specific electrophoresis bands by both single PCR assay and multiplex PCR assay,and the fragments were gained respectively,that was 732 bp for MG,207 bp for MS and 850 bp for MM. While the same bands were gained on agarose gel, the fluid cultures containing Mycoplasma were directly performed for PCR. These data showed that the established multiplex PCR assay might facilitate differentiation of clinical infection by different serotype of avian Mycoplasma. At the same time,the comparison test between ELISA for serum and PCR for MG in chickens also presented the availability of detecting MG clinically.

Key words: avian Mycoplasma; diagnosis; PCR; ELISA