›› 2011, Vol. 38 ›› Issue (8): 221-224.

• 经验交流 • Previous Articles     Next Articles

Study on Inactivating Effect of 60Coγ Rays on Newcastle Disease Virus, H9N2 AIV and PRRSV

XU Shou-zhen1, WANG Xin2, YIN Yan-bo1   

  1. 1. College of Animal Science and Veterinary Medicine,Qingdao Agricultural University,Qingdao 266109,China;2. Qingdao Olandbetter Bioengineering Co. Ltd., Qingdao 266101,China
  • Received:1900-01-01 Revised:2011-06-14 Online:2011-08-20 Published:2011-08-20

Abstract: NDV,H9N2 AIV and PRRSV in different volumes at different states were irradiated by 60Coγ ray and the inactivating effects of 60Coγ rays on these viruses in inactivated vaccine were investigated. The results showed that NDV,H9N2 AIV and PRRSV in the volume of 5000 and 10000 mL at frozen or liquid state were not inactivated by 6.0 kGy 60Coγ irradiation respectively. After irradiation the HA titers of NDV and AIV H9N2 contained in frozen allantoic fluid were lower than those of unirradiated frozen allantoic fluid while the HA titers of NDV and AIV H9N2 were higher than those of unirradiated allantoic fluid. At liquid state 250 mL PRRSV in cell culture was completely inactivated by 60Coγ rays,at the same time NDV and H9N2 AIV in the volume of 250,5000 and 10000 mL were not completely inactivated,and the HA titer of 250 mL NDV was significantly different(P<0.05) compared with that of NDV in the volume of 5000 and 10000 mL. 60Coγ ray irradiation has a good inactivating effect on PRRSV at liquid state and it can be used to inactivate viruses in inactivated vaccine.

Key words: NDV; H9N2 AIV; PRRSV; 60Coγ ray; inactivation

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