Abstract: The aim of present study is to establish a real-time PCR for quantitative analysis of mouse β-actin mRNA expression. A set of primer was designed according to the mouse β-actin mRNA sequence in GenBank. And a SYBR Green Ⅰ-based reverse real-time PCR (RRT-PCR) was successfully developed. The results showed that RRT-PCR assay established had a high amplification efficiency(97.7%),wide linear range(9.3×10² to 9.3×10⁷ copies/reaction),good reproducibility and specificity. The detection limit was 9.3 copies/reaction. The results provided a basis for use of β-actin as a reference gene in gene expression analysis in mouse.

Key words: mouse; β-actin gene; real-time RT-PCR