细菌素Bacteriocin E50-52(H)基因设计 及其毕赤酵母表达载体构建

Abstract

Abstract: Bacteriocin E50-52 gene was designed according to its amino acid sequence and cloning site of constitutive secreted plasmid pGAPZαA,then synthesized,inserted into pMD18-T. Recombinant pGAPZα- Bacteriocin E was constructed by ligating the digested target gene and pGAPZαA with XhoⅠ and XbaⅠ. Recombinant Pichia pastoris was constructed by transforming linearized recombinant plasmid in strain SMD1168. Sequencing result of recombinant vector and genome of Pichia pastoris indicated this expression plasmid was successfully constructed.

Key words: Bacteriocin E50-52; gene design; Pichia pastoris; constitutive expression vector

CLC Number:

YU Zhan-qiao;MA Qing-shan;HAN Bing;ZHANG Ri-jun. Gene Design of Bacteriocin E50-52 and Construction of its Pichia pastoris Expression Vector[J]. , 2011, 38(4): 77-79.

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Gene Design of Bacteriocin E50-52 and Construction of its Pichia pastoris Expression Vector

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