猪繁殖与呼吸系统综合征病毒(PRRSV) CH-IR株单克隆抗体的制备

Abstract

Abstract: The Balb/c mouse were immunized for several times with the purified PRRSV,the mouse spleen cells immunized were fused with SP2/0 cells. By indirect enzyme linked immunity assay and four 4 sub-cloning ,we got one monoclonal antibody hybridoma cells line(3D₆), it was the sub-type of IgG2a by identification, and the chromosome number was 99. The cells supernatant titer and ascites were 1∶512 and 1∶20480. This McAb did not occurred cross reaction with PRV, PPV and HCV. and showed excellent specificity. It could secret antibody stably for 20 generation. The McAb could be used to detect PRRSV antigen and would be useful for studying diagnostic methods of PRRSV.

Key words: PRRSV; ELISA; monoclonal antibody

CLC Number:

LAN Hai-nan;KONG Jin-chao;ZHAO Xiao-li;YAN Feng;LI Wei;ZHAO Xue;GUO Feng;ZHENG Xin. Preparation of Monoclonal Antibody of PRRSV (CH-IR Strain)[J]. China Animal Husbandry & Veterinary Medicine, 2011, 38(4): 74-76.

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Preparation of Monoclonal Antibody of PRRSV (CH-IR Strain)

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