Abstract: Liver, spleen, heart blood were collected from died Chrysolophus pictus by sterile method, and then cultured on medium to isolate pathogenic bacteria. The isolated bacterium was identified by biochemical test and its pathogenicity was confirmed by the animal challenge experiment. The isolate 16S rRNA gene was amplified by polymerase chain reaction technology and its nucleotide sequence was determined. Homology comparison with gene sequence in the GenBank was carried out and the phylogenetic tree was constructed by the DNAStar. The result showed a strain Escherichia coli was isolated and the bacteria could made the mice died. The 16S rRNA gene of the bacterium shared 99.9% homology in the nucleotide sequence with the other E.coli in the GenBank. The Chrysolophus pictus died from the virulent E.coli.

Key words: Chrysolophus pictus; colibacillosis; bacteriodiagnosis