氯羟吡啶抗原合成和多克隆抗体制备

Abstract

Abstract: In this study, a clopidol hapten was synthesized, in which a carboxylic branch was attached to the phenolichydroxyl of clopidol. The structure was confirmed with MS. Then the artificial antigens were synthesized using active ester reaction and the isobutyl chloroformate method. The coupling ratio and antigen concentration were further determined by UV/VIS spectrometer. After then the anti-serum was produced and the specificity and sensitivity were studied, which was expected to provide reference for the establishment of ELISA method for clopidol.

Key words: clopidol; antigen; polyclonal antibody; ELISA 

CLC Number:

S859.84

FENG Cai-wei;GUO Xin. Synthesis of Clopidol Artificial Antigen and Development of its Polyclonal Antibody[J]. , 2011, 38(4): 173-176.

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Synthesis of Clopidol Artificial Antigen and Development of its Polyclonal Antibody

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