Abstract: 119 suffork mutton were superovulated,include 48 were first superovulated sheep and 71 used again. The results showed that the average recovered embryos of second superovulated sheep was significantly lower than first superovulated donors(P＜0.05), The number of corpus luteum was not significant different by the first and second blunt donors(P＞0.05),both the available rate embryo of was not significant different (P＞0.05). The synchronization of estrous through the second estrous cycle or vaginal plug methods,achieved the desired results,but both the average recovery embryos of second superovulated sheep were significantly lower than first superovulated donors (P＜0.05).

Key words: Suffork mutton; superovulation; flushing embryo from oviduct; embryo transplantation