›› 2011, Vol. 38 ›› Issue (3): 108-111.

• 生物技术 • Previous Articles     Next Articles

Generation of Anti-PRV gC Antibodies and Analysis of PRV gC Expression

LIU Qing-wei1,2，QIU Ya-feng1，WANG Xiao-du1，GUO Lin1，LIU Chao1,SHEN Yang1，LI Xiang-dong1，SHAN Hu2，MA Zhi-yong1   

  1. （1.Department of Veterinary Public Health，Shanghai Veterinary Research Institute，Chinese Academy of Agricultural Sciences，Shanghai 200241，China; 2.College of Animal Science and Technology，Qingdao Agricutural University，Qingdao 266109，China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-03-20 Published:2011-03-20
  • Contact: MA Zhi-yong

Abstract: The purified prokaryotic expression of PRV gC functional domain named His-gCN813 was used as immunogen to generate antibodies，and the PRV gC expressed in eukaryotic cell was analysised. The full length of PRV gC was cloned from PRV genome to construct recombinant plasmids p3xFLAG-gC for eukaryotic expression. The expression of PRV gC in p3xFLAG-gC transfected cells and PRV infected cells were analyzed with the anti-gC antibodies by Western blotting and immunofluorescence assay. The results indicated that the eukaryotic expressed system of PRV gC were constructed，gC specific poly-clonal antibodies were generated，detecting 72 ku gC expressed in p3xFLAG-gC transfected cells and 55，72 and 94 ku gC expressed in PRV infected cells，mainly located in the cytoplasm by immunofluorescence. PRV gC would be a potential molecular indicator of PRV，laid foundation for the future research of PRV replication and interaction between PRV and host.

Key words: pseudorabies virus; anti-PRV gC antibody; Western blotting

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