小鼠卵巢颗粒细胞的优化培养

›› 2011, Vol. 38 ›› Issue (2): 107-110.

• 生物技术 • Previous Articles Next Articles

Optimization of Mouse Granulosa Cells Culture

YANG Yong-mei, CHEN Juan, CHEN Yang, JIANG Huai-zhi

(Department of Animal Science and Technology，Jilin Agricultural University，Changchun 130118，China)

Received:1900-01-01 Revised:1900-01-01 Online:2011-02-20 Published:2011-02-20
Contact: JIANG Huai-zhi

Abstract

Abstract: The purpose of granulosa cells in mice to find a suitable environment for the growth in vitro. Respectively, separated by experimental mouse ovarian granulosa cells after serum RMPI1640, with 10% serum RMPI1640, serum DMEM (high glucose) , with 10% serum DMEM (high glucose) and containing 15% serum DMEM (high glucose) cultured in this study. The results showed that DMEM (high glucose) culture was generally better than RMPI1640, and containing 15% serum DMEM (high glucose) was an ideal medium.

Key words: granulosa cells; serum concentration; DMEM (high glucose); RMPI1640

CLC Number:

Q813

YANG Yong-mei;CHEN Juan;CHEN Yang;JIANG Huai-zhi. Optimization of Mouse Granulosa Cells Culture[J]. , 2011, 38(2): 107-110.

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