Abstract: Predicting and analysising the properties of HP-PRRSV BJSD and BJPG sequence,which will prepare for further study of this strain. Molecular biology software and server are used to analysis the complete sequence of HP-PRRSV BJSD and BJPG strain,the biochemistry properties,solubility of various structural proteins and nuclear location singal (NLS). The results show that there are 12 non-structural and 6 structual protein at least in HP-PRRSV BJSD and BJPG. When we compare different protein homology,we found that GP3 and GP5 are high mutant,M and N are conservative. The protein glycosylation site of GP3,GP5 and M are different from the reference strains,but GP2,GP4 and N are the same. Server shows that biochemical characteristics of GP2,M and N protein of BJSD and BJPG strains is identical,the ratio of alkaline amino acid of N is higher than GP2 and M,and the total average hydrophilic value obviously lower than other proteins. GP2,GP3 and GP4 are unstable; GP5,M and N are opposite. The solubility of all proteins of BJSD and BJPG strains is consistent but GP3 and GP5 has somewhat different. Prediction of 6 structural protein insoluble probability are accounted for more than 66 percent,GP3 is highest. The nuclear localization of structural proteins of BJSD and BJPG strains show that distribution of all proteins is identical but GP3 and GP5. The properties of above strains can pave the way for the diagnosis and treatment of PRRS.

Key words: highly pathogenicity; porcine reproductive and respiratory syndrome(PRRS); sequence analysis; BJSD strain; BJPG strain