Abstract: The canine distemper virus (CDV) isoladed from lesser panda and domesticated by the laboratory of genome sequence determination,genetic characteristics and genetic stability of the H gene. According to full-length genome sequence of canine distemper virus published in GenBank, 17 pairs of specific primers were designed and synthesized to amplify full-length cDNA as template with total RNA of lesser panda canine distemper virus, using the method of RT-PCR, and cloned into pEASY-Blunt Simple vector for determination of full-length cDNA sequences.The results showed that the homology of whole genome sequence of lesser panda source canine distemper virus and 12 different strains AF014953, AY445077, AY542312, AY466011, AY386316, AF164967, EU716337, EU726268, AY443350, AB474397, GU138403 and AY649446 is 86.6%, 92.4%, 92.5%, 92.5%, 94.3%, 96.3%, 95.9%, 87.1%, 94.5%, 92.3%, 87.4% and 94.6%, respectively. It had the closest genetic relationship with the standard virulent strain A75/17, gene homology was 96.3%, but compared the vaccine strain Onderstepoort and it had distant genetic relationship, only 86.6% homology.Phylogenetic analysis of H gene of lesser panda source canine distemper virus and other thirty CDV published in GenBank in different districts showed that, canine distemper virus of lesser panda source belonged to the AsiaⅠ, the potential glycosylation sites of H protein gene were formed by amino acid from 309 to 311 of H protein, the wild strains had it common but the vaccine strain didn't, and may be associated with the virus immunogenic. Therefore, attenuated panda source CDV in the prevention of immune targeted may be better than the existing vaccines strain.

Key words: canine distemper virus; genomic sequence determination; sequence analysis