几种佐剂对肌肉注射猪支原体肺炎活疫苗免疫刺激能力的增强作用研究

›› 2011, Vol. 38 ›› Issue (10): 163-168.

• 疾病防治 • Previous Articles Next Articles

Immune Study of a Intramuscular Injected Live Vaccine against Mycoplasma hyopneumoniae Enhanced by Different Adjuvants

XIONG Qi-yan¹, FAN Zhi-peng^1,2, GAN Yuan¹, KONG Meng¹, FENG Zhi-xin¹, LIU Mao-jun¹, SHAO Guo-qing¹

1. Key Laboratory of Animal Diseases Diagnostic and Immunology,Ministry of Agriculture,National Center for Engineering Research of Veterinary Bio-products,Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2. Graduate College of Shanxi Agricultural University,Taigu 030801, China

Received:2011-01-28 Revised:1900-01-01 Online:2011-10-20 Published:2011-10-20

Abstract

Abstract: To achieve the intramuscular injection of the attenuated live vaccine against Mycoplasma hyopneumoniae,different adjuvants were applied to enhance the immunogenicity of the vaccine. Cellular and humoral immune response was detected after the immunization. The results showed that ISCOM-matrix,as well as the mixture of levamisole and chitosan,effectively increased the proliferation of PBMC and the level of serum IgG antibody. The mixture of levamisole and astragalus polysaccharide only enhanced the cellular immune response while the antibody level showed no change. No obvious enhancement was detected in saponin adjuvant group. These data would help the subsequent researches of the intramuscular inoculating method of this live vaccine.

Key words: adjuvant; live vaccine; mycoplasmal pneumonia of swine

CLC Number:

S851.3

XIONG Qi-yan;FAN Zhi-peng;GAN Yuan;KONG Meng;FENG Zhi-xin;LIU Mao-jun;SHAO Guo-qing. Immune Study of a Intramuscular Injected Live Vaccine against Mycoplasma hyopneumoniae Enhanced by Different Adjuvants[J]. , 2011, 38(10): 163-168.

[1]	HAN Bing, TANG Siqi, ZHU Dawei, LI Hailing, FENG Yali, SU Chaofan, XU Yina, LENG Haoyu, WANG Yongtao, ZHANG Yuemei, TAI Xiaochun, ZHANG Ying. Research Progress of Novel Vaccine Adjuvants [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2460-2467.
[2]	LIU Xinyue, LI Lifang, WANG Zhenbao, ZHANG Wenliang, ZHANG Xin, ZHAO Hongtao, LI Pengyu, ZHENG Haotian, XIAO Jin, QI Peng. Adjuvants Screening and Immune Protection of Porcine Circovirus Type 2 Synthetic Peptide Vaccine [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1068-1080.
[3]	LIU Hongying, WANG Jingyu, CUI Zhenzhen, MA Qingxia, WANG Tingting, LI Jianming, SHI Kun, GONG Qinglong, DU Rui, LENG Xue. Research Progress on Immune Adjuvant Effect and Related Mechanism of Active Ingredients of Traditional Chinese Medicine on Animal Vaccines [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 390-397.
[4]	SHI Qian, XU Xinfeng, LI Xuewen, XIAO Yuanyuan, BAI Meihua, ZHANG Fuchun. Research Progress on the Mechanism of Polysaccharide as Adjuvant of Veterinary Vaccine [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1895-1907.
[5]	WAN Meng, CHEN Yilin, QIAN Yuhao, HUANG Xuan, XIA Lianchen, WANG Chunwei, ZHU Aixia. Preparation of Cationic Squalene Liposome Adjuvant and Physical and Chemical Properties [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1431-1439.
[6]	PAN Yue, CAO Qi, REN Honglin, HU Pan, LI Yansong, ZHOU Yu, LIU Zengshan, LU Shiying. Prokaryotic Expression of Murine CD40L and Its Immunopotentiating Effect on Tetrodotoxin Hapten [J]. , 2019, 46(8): 2438-2445.
[7]	CHENG Yao, MAO Li-ping, WEN Zhao-hai, Gulmayra Kare, JIAN Zi-jian, ZHAI Shao-hua. Screening of Immunoadjuvant Compound and Bait of Animal Rabies Oral Vaccine and Their Influence on Immune Effects [J]. , 2017, 44(2): 546-553.
[8]	CHANG Jiang-yan, LI Zhao, FEI Shao-jiang, YANG Jian-fa, ZOU Feng-cai. Application of Nanotechnology in Parasitology [J]. , 2017, 44(11): 3294-3299.
[9]	LI Shi-fang, LIU Ze-zhong, CHANG Hui-yun. Research Progress on Cytokine Adjuvants [J]. , 2017, 44(1): 296-301.
[10]	XU Xin-ting, SHI Kai-chuang, LI Chang-ting, LONG Fei-xiang, MO Sheng-lan, CHEN Han-zhong. Analysis of the Immune Efficacy of Attenuated Live Vaccine in the Pig Herds Previously Infected with Wild-type Porcine Reproductive and Respiratory Syndrome Virus [J]. , 2016, 43(9): 2447-2454.
[11]	LONG Chong-chong, DENG Wei-xi, MAO Yi-zhi, ZENG Gui-ying, WU Bo-tao, XIONG Chao-li, XU Mao-wen, XIA Peng, WEN Ming. Construction of Recombined Plasmid with OmpA Gene of Goat Chlamydophila abortus and Immunogenicity Effect in Mice [J]. , 2016, 43(11): 2886-2891.
[12]	WANG Li-bo, WU Run-sheng, YAN Chao, LV Tun. Effects of Different Adjuvants on the Immunogenicity of PCV2-Cap Protein [J]. , 2016, 43(11): 3037-3046.
[13]	LIANG Kong-xian, LV Xiao-jun, DING Dan, XU Qi, ZHOU Jie, LIU Wei. Preparation of Adjuvant Diluent for Live Vaccine against Classical Swine Fever and Evaluation of its Immune Effects [J]. , 2015, 42(9): 2467-2473.
[14]	LIU Hao-peng, WU Feng-ming, HU Jing-jing, HAN Jing-fang, PEI Zhang-fu, TANG Qin, TANG Man-hua, CHEN Rui-ai, HE Dong-sheng. Research of Virus Persistence and Antibody Dynamic in the Blood after Immune in Intensive Farms with Different Strains of PRRS Live Vaccine [J]. , 2015, 42(1): 197-202.
[15]	GONG Lei, ZHOU Zheng-hong, ZHOU Jun-ping, YAN Ting-liang. Research Progress on Natural Immunomodulatory Medicine for Tumor Adjuvant Therapy [J]. , 2014, 41(7): 247-251.

Immune Study of a Intramuscular Injected Live Vaccine against Mycoplasma hyopneumoniae Enhanced by Different Adjuvants

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments