›› 2011, Vol. 38 ›› Issue (1): 72-75.

• 生物技术 • Previous Articles     Next Articles

Developement of Monoclonal Antibody against Bovine Interferon-γ and Establishment of ELISA Detection Method

LI Chao,LIN Xiang-mei,MEI Lin,LI Quan-fen,HAN Xue-qing   

  1. (Chinese Academy of Inspection and Quarantine,Beijing 100029, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-01-01 Published:2011-01-01
  • Contact: HAN Xue-qing

Abstract: Establish bovine tuberculosis BovIFN-γ diagnostic method. Balb/c mice were immunized 5 times with purified reccombinant BovIFN-γ protein, and the McAb against BovIFN-γ was prepared by hybridoma technique, then a double antibody sandwich ELISA method was developed based on the McAb and polyclonal antibody, and further optimized the reaction conditions. Finally, a hybridoma which stably secreted specific monoclonal antibody against BovIFN-γ was named A12E9. The titer of McAb was 1∶107, and the McAb belonged to IgG1 subclass. The sensitivity of double antibody sandwich ELISA method was 40 ng/mL, with good specificity. This method provides a basis for furth researches on the bovine tuberculosis BovIFN-γ diagnostic method.

Key words: BovIFN-γ; monoclonal antibody; double antibody sandwich ELISA

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