Abstract: In order to evaluate the effect of co-culturing with human granule cells on the IVM of bovine oocytes,we divided the bovine oocytes into the experimental group and the control group．In the experimental group, bovine oocytes were co-culturing with human granule cells in the IVM medium. While in the control group bovine oocytes were cultured in the same IVM medium. The rates of maturating and blastocyst formation rates through parthenogenetic activation were compared between the two groups. The maturating rates in the experimental group (72.35%) was higher than that in the control group(52.86%) (P＜0.01). After parthenogenetic activation, the blastocyst formation rates in the two groups were 12.82％ and 4.76％, but the differences were not significant（P＞0.05）. The results suggested that human granule cells can be used for co-culturing in the IVM of bovine oocytes, co-culturing with human granule cells may elevate the nuclear maturing rates.

Key words: human granule cells; bovine oocyte; in vitro maturation〖