›› 2018, Vol. 45 ›› Issue (8): 2247-2254.doi: 10.16431/j.cnki.1671-7236.2018.08.025

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Effects of Gallic Acid on in vitro Maturation of Bovine Oocytes

HUANG Yongjun, LI Xiaofeng, WEN Dongmei, SHE Chun, DENG Kai, PANG Erke, SHAO Qiming, SHI Deshun, WEI Yingming, LU Fenghua   

  1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning 530000, China
  • Received:2018-01-02 Online:2018-08-20 Published:2018-08-15

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Abstract:

The aim of this study was to investigate the effects of adding various concentrations of gallic acid (GA) to the maturation medium on the maturation of bovine oocytes and early embryo development in vitro in order to further optimize culture system of bovine oocytes in vitro maturation.Oocytes were matured in vitro on culture medium with different concentrations (0,10,30,50,100 μmol/L) of GA in incubator for 22 to 24 h.Subsequently,the maturation rate of oocyte and the expansion of cumulus cell were calculated,and the cleavage rates,the blastocyst rates,the number of blastomere and the apoptosis rate of blastomere were evaluated after in vitro fertilization (IVF).According to the above test results,oocytes were matured in vitro on culture medium with the optimal concentration of GA for 24 h,and then the intracellular reactive oxygen species (ROS) level and total glutathione level were detected.The results showed that the mature medium with 30 μmol/L GA could significantly increase the oocytes maturation rates and the sore of expansion of cumulus cell than those of the control group (P<0.05),but there was no significant difference between other treatment groups and control group (P>0.05).The cleavage rates of 10 and 30 μmol/L GA groups were significantly increased than 0 and 100 μmol/L GA groups(P<0.05),while there were no significant differences among 0,50,100 μmol/L GA groups (P>0.05).The blastocyst rate of 30 and 100 μmol/L GA groups were significantly improved than that of control group (P<0.05),but 10 and 50 μmol/L GA groups had no significant differences with control group (P>0.05).The No.of blastomere in 30 and 100 μmol/L GA groups were significantly higher than that of the control group (P<0.05),however,the apoptosis rate of blastomere in each group had no significant difference (P>0.05).The detection of the ROS level and total glutathione content in oocyte showed that the levels of ROS were significantly decreased and the content of total glutathione increased significantly in 30 μmol/L GA group (P<0.05).These results suggested that adding GA during IVF might reduce oxidative stress by decreasing ROS levels directly and increasing total glutathione levels in oocytes,thus improve the quality of oocyte maturation and its subsequent IVF embryos developmental ability.

Key words: gallic acid (GA); in vitro fertilization (IVF); bovine oocytes

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