美洲狮和亚洲黑熊蛔虫ITS序列扩增及序列分析

›› 2010, Vol. 37 ›› Issue (8): 41-44.

• 生物技术 • Previous Articles Next Articles

PCR Amplification and Sequence Analysis of Internal Transcribed Spacers(ITS) of Ascarids from Puma concolor and Ursus thibetanus

HE Xian-hui¹，CHEN WU²， HE Le-tian^1，3，WANG Pei-yuan¹，YUAN Zi-guo¹，LIN Rui-qing¹

(1.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; 2.Guangzhou Zoo, Guangzhou 510070, China; 3.Guangzhou Chuanglai Biotechnology Limited Company, Guangzhou 510640, China)

Received:1900-01-01 Revised:1900-01-01 Online:2010-08-20 Published:2010-08-20
Contact: LIN Rui-qing

Abstract

Abstract: The DNA internal transcribed spacer (ITS) and 5.8S rDNA of ascaris from the Puma concolor and the Ursus thibetanus in Guangzhou zoo were amplified by PCR respectively using a pair of conserved primers and the amplicons were cloned into pGEM-T Easy vector. The positive clones identified by PCR amplification were sequenced and analyzed, and compared with relevant sequence in GenBank^TM. The results revealed that the ITS and 5.8S sequence of the ascaris from Puma concolor and the ITS-2 sequence from Ursus thibetanus were significantly similar with that of Toxascaris leonina and Baylisascaris transfuga available in GenBank^TM respectively. We concluded that the ascaris from Puma concolor represent the T. leonina, and that from Ursus thibetanus represent the B. Transfuga.

Key words: Puma concolor; Ursus thibetanus; ascaris; ribosomal DNA internal transcribed spacers; sequence analysis

CLC Number:

S852.73

HE Xian-hui;CHEN WU;HE Le-tian;WANG Pei-yuan;YUAN Zi-guo;LIN Rui-qing. PCR Amplification and Sequence Analysis of Internal Transcribed Spacers(ITS) of Ascarids from Puma concolor and Ursus thibetanus[J]. , 2010, 37(8): 41-44.

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PCR Amplification and Sequence Analysis of Internal Transcribed Spacers(ITS) of Ascarids from Puma concolor and Ursus thibetanus

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