Abstract: Cow mastitis is one of the most common diseases of dairy cow caused by a variety of pathogenic infection, which not only cause significant economic losses, but also affects the quality of milk, endangering people’s health. Conventional detection methods in mastitis are present in varying degrees of limitations. This paper reviewed the PCR, multiple PCR, real-time PCR, gene chips and other molecular biology techniques diagnostic studies in dairy cow mastitis, and analyzed their development prospects.

Key words: dairy cow; mastitis; molecular diagnostics