5株堆形艾美耳球虫线粒体cox3基因的扩增及序列分析

›› 2010, Vol. 37 ›› Issue (6): 63-66.

• 生物技术 • Previous Articles Next Articles

PCR Amplification and Sequence Analysis of Mitochondrial Cytochrome c Oxidase Subunit Ⅲ (cox3) of five Eimeria acervulina Strains

XIE Wen-qin1, TAN Zhi-jian2, FU De-wen2, QIU Li-ling3, WENG Ya-biao3,LIU Yi1, LIN Rui-qing3, XU Min-jun3

(1.College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China;2.Foshan Standard Bio-Tech Co. Ltd., Foshan 528138, China;3.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China)

Received:1900-01-01 Revised:1900-01-01 Online:2010-06-20 Published:2010-06-20
Contact: XU Min-jun

Abstract

Abstract: To study genetic variation of mitochondrial cytochrome c oxidase subunit Ⅲ (cox3) gene of Eimeria acervulina, the cox3 was amplified by polymerase chain reaction (PCR) and 572 bp of partial cox3 (pcox3) was obtained from five strains of E. acervulina. By sequence comparison and comparative analysis with the cox3 gene of some protozoa of Phylum Apicomplexa avalidable in GenBank, the results showed that there was no difference in the nucleotides of the pcox3 sequences obtained from five strains of E. acervulina, and there were distinct differences in the nucleotides compared with other protozoa of Phylum Apicomplexa. The sequence diferences were 51% and 50% compared with Plasmodium and Leucocytozoon respectively, and was less than 50% compared with Theileria. It was the first time that cox3 of E. acervulina was reported. The results of the present study provided a foundation for further studies of population genetics of Eimeria.

Key words: Eimeria acervulina; mitochondrial DNA; cox3; sequence analysis

CLC Number:

S852.72

XIE Wen-qin;TAN Zhi-jian;FU De-wen;QIU Li-ling;WENG Ya-biao;LIU Yi;LIN Rui-qing;XU Min-jun. PCR Amplification and Sequence Analysis of Mitochondrial Cytochrome c Oxidase Subunit Ⅲ (cox3) of five Eimeria acervulina Strains[J]. , 2010, 37(6): 63-66.

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PCR Amplification and Sequence Analysis of Mitochondrial Cytochrome c Oxidase Subunit Ⅲ (cox3) of five Eimeria acervulina Strains

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