Abstract: Using chromosome DNA of Escherichia coli MG1655 as template, sodC gene was amplified by PCR and purified by purification kit in this study. The PCR product of sodC gene was cloned into pMD18-T vector and transformed into DH5α competent cells. The recombinant plasmid containing sodC gene was selected and the inserted sodC gene was identified by cleaved with restriction endonuclease Sac I and Kpn I, and following by PCR amplification and sequencing. The results showed that the whole sodC gene was cloned successfully. Sequence analysis indicated that sodC gene displayed 99.6% nucleotide identities with the published sequences by GenBank. This research proved a foundation for the construction of recombinant expression vector of sodC gene.

Key words: superoxide dismutase; cloning; sequence analysis