猪圆环病毒2型、猪伪狂犬病病毒、猪细小病毒多重PCR检测方法的建立

Abstract

Abstract: Abstract：According to the gene sequences in GenBank of porcine circovirus type 2 （PCV2）, porcine pseudorabies virus （PRV） and porcine parvovirus （PPV）, three pairs of specific primers were designed for amplifying the four specific fragments of PCV2, PPV and PRV 350 bp 270 bp and 191 bp in length, respectively. The results indicated that multiplex PCR is both sensitive and specific, providing a new method for the clinical diagnosis and epidemiological research of the three diseases.

Key words: Key words：porcine circovirus type 2; pseudorabies virus; porcine parvoviruses; multiplex PCR

ZHAO Yan-zong; HE Dong-sheng;ZHANG Yan-ping;SU Dan-ping. Establishment of a Multiplex PCR Assay for Simultaneous Detection of PCV2, PPV and PRV[J]. China Animal Husbandry & Veterinary Medicine, 2010, 37(2): 80-82.

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Establishment of a Multiplex PCR Assay for Simultaneous Detection of PCV2, PPV and PRV

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