Abstract: In this study, a pair of special primers(MT-Ⅲ_SP1 and MT-Ⅲ_SP2) were designed for cloning the coding sequence of metallothionien-Ⅲ（MT-Ⅲ） from brain tissue in goat. The MT-Ⅲ coding sequence in goat were 207 bp. The goats MT-Ⅲ gene sequences had been submitted to GenBank under accession numbers EF471976.Goat MT-Ⅲ gene coded 68 amino acids, including 19 Cys residues. Goat MT-Ⅲ had characteristic MDPET，C-X-C，C-C-X-C-C，C-X-X-C and KKS sequences of MTs, where X was non-cysteine amino acids. The amino acids sequences of goat shared 99.5%，98.6%，89.4%，88.5%，88.2%，88.2% and 83.8% sequence similarity with cattle, sheep, dog, pig, human, rhesus monkey and mouse, it showed that the MT-Ⅲ was very conservative.The phylogeny of MT-Ⅲ from nucleotide and amino acids sequence were same, and was also consistent with the result of morphology and physiology.

Key words: goat; metallothionien-Ⅲ; cDNA; sequence analysis