›› 2010, Vol. 37 ›› Issue (12): 226-228.

• 经验交流 • Previous Articles     Next Articles

The Research of Locating DON in Liver with Streptavidin-perrosidase Method

LIU Ben-jie1,NIU Geng-zhi1,HE Min2,LU Pei-cheng1,ZHANG Man-yu1,QIAN Hui-hui1,MA Hong-na1,DENG Xian-bo1   

  1. (1. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;2. Gongzhou Vocational College of Technology and Business, Guangzhou 510430, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-12-20 Published:2010-12-20
  • Contact: DENG Xian-bo

Abstract: Deoxynivalenol (DON) contamination of grains is a worldwide phenomenon. Pig is the most susceptible animal to DON. In this research, 10 healthy clean pigs were divided into 2 groups randomly. One group was given DON from breast vein with a dose of 75 μg/kg b.w.,while the other group using sodium chloride. 30 min after the intravenous(IV) administration, pigs were killed and liver tissue samples were taken out quickly. Streptavidin-perrosidase(SP) method was used to detect the DON in liver. The positioning study of SP method showed that DON could be seen in liver’s portal area(interlobular veins). This research studied the distribution of deoxynivalenol in organs and tissues of pig, offering the source for the research of residue of toxin.

Key words: DON; liver; pig; SP method

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