›› 2010, Vol. 37 ›› Issue (10): 92-96.

• 生物技术 • Previous Articles     Next Articles

Culture and Identification of Primordial Germ Cells from Inner Mongolia Cashmere Goat

LIU Yi-yi1，ZHAO Li-xia1，LI Yan4，BAI Jun2，ZHOU Huan-min3   

  1. （1.College of Life Science，Inner Mongolia Agricultural University， Hohhot 010018， China；2.College of Animal Science,Inner Mongolia Agricultural University， Hohhot 010018， China；3.Science and Technology Agency， Inner Mongolia Agricultural University， Hohhot 010018， China； 4.The Key Laboratory of Mammalian Reproductive Biology and Biotechnology of the Ministry of Education，Inner Mongolia University， Hohhot 010021， China）
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-10-20 Published:2010-10-20
  • Contact: ZHOU Huan-min

Abstract: In this study,we cultured and identified of primordial germ cells (PGCs) which were isolated from genital ridge of the Inner Mongolia cashmere goat fetus. Three groups (A,B,C) culture medium were detected establish culture system for culture of primordial germ cells from Inner Mongolia cashmere goat. After identified by RT-PCR, AKP staining and immunofluorescence,the results showed that the RT-PCR detection of β-actin, hTERT, GAPDH, Nanog and Oct3/4, AKP identified was positive, immunofluorescence detection of Oct3/4, SSEA-3, SSEA-4 were positive. Group A culture medium without any factors could only be passaged 1 generation, group B culture medium supplemented with LIF and insulin could be passaged 4 generations, group C culture medium supplemented with LIF, insulin and high-quality fetal bovine serum culture system could be passaged 9 generations.

Key words: primordial germ cells; Oct3/4; immunofluorescence; Nanog〖

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