K亚群禽白血病病毒gp85单因子血清的制备及其特异性鉴定

doi:10.16431/j.cnki.1671-7236.2016.02.012

Abstract

Abstract: This study was to prepare a diagnostic serum to rapidly diagnose subgroup K avian leukemia virus (ALV-K).The ALV-K were inoculated in DF1 cells and extracted DNA from infected cells to amplify ALV-K-gp85 (1 005 bp) and ALV-K-gp85 (fragment) (510 bp) genes by PCR.Their correct reading frames were inserted into pET-28a(+) expression vector,then transformed into BL21 (Rosetta) strain.The expressed proteins were used to immune Kunming White mice to prepare antiserum.Recombinant fusion proteins of 39.85 and 22.01 ku were successfully obtained and showed good immunogenicity.Indirect immunofluorescence assay (IFA) showed that the two sera could react with ALV-K,but could not react with ALV-A,ALV-B and ALV-J.The experiment prepared the single factor serum that could be used to specifically diagnosis subgroup K avian leukemia for the first time,at the same time,the single factor serum laid the foundation for rapid diagnoses of subgroup K avian leukemia.

Key words: subgroup K avian leucosis virus; gp85 gene; expression; indirect immunofluorescence assay (IFA); single factor serum

CLC Number:

S852.4⁺3

SUN Peng, CHEN Zi-meng, ZHAO Guo-liang, CUI Ning, ZU Ming, CUI Zhi-zhong, SU Shuai. Preparation and Specific Identification of Single Factor Serum of Subgroup K Avian Leukosis Virus gp85[J]. , 2016, 43(2): 371-376.

References

[1] 卡尔尼克B W.禽病学[M].北京:中国农业出版社,1999.
[2] Payne L N,Brown S R,Bumstead K,et al.A novel subgroup of exogenous avian leukosis virus in chickens[J].J Gen Virol,1991,72:801-807.
[3] Payne L N,Howes K,Gillespie A M,et al.Host range of Rous sarcoma virus pseudo type RSV (HPRS-103) in 12 avian species:Support for a new avian retrovirus envelope subgroup designates J[J].J Gen Virol,1992,73:2995-2997.
[4] 王鑫,赵鹏,崔治中.我国地方品种鸡分离到的一个禽白血病病毒新亚群的鉴定[J].病毒学报,2012,28(6):609-614.
[5] 钟兴福,王培坤,杨永立,等.广西地方禽类品种ALV和REV感染情况的调查及ALV分离株gp85基因序列分析[J].中国畜牧兽医,2015,42(7):1647-1653.
[6] 张志,崔治中,赵宏坤,等.商品代肉鸡J亚群禽白血病的病理及病毒分离鉴定[J].中国兽医杂志,2002,38:6-8.
[7] Cui Z,Du Y,Zhang Z,et al.Comparison of Chinese field strains of avian leukosis subgroup J viruses with prototype strain HPRS-103 and United States strains[J].Avian Dis,2003,47:1321-1330.
[8] Zhang Q C,Zhao D M,Guo H J,et al.Isolation and identification of a subgroup a avian leukosis virus from imported meat-type grand-parent chickens[J].Virol Sin,2010,25(2):130-136.
[9] Xu B,Dong W,Yu C,et al.Occurrence of avian leukosis virus subgroup J in commercial layer flocks in China[J].Avian Pathol,2004,33:13-17.
[10] 王辉,崔治中.蛋鸡J亚群白血病病毒的分离鉴定与序列分析[J].病毒学报,2008,24:369-375.
[11] Sun S H,Cui Z Z.Epidemiological and pathological studies of subgroup J avian leukosis virus infectious in Chinese local "yellow" chicken[J]. Avian Pathol,2007,36:221-226.
[12] 成子强,张利,刘思当,等.中国麻鸡中发现禽J亚群白血病[J].微生物学报,2005,45:584-587.
[13] 刘绍琼,王波,张振杰,等.817肉杂鸡肉瘤组织分离出A、J亚禽白血病病毒[J].畜牧兽医学报,2011,42(3):396-401.
[14] 倪伟,秦立廷,孙洪磊,等.某海兰褐鸡场祖代、父母代及商品代鸡血管瘤型ALV-J分离与鉴定[J].畜牧兽医学报,2011,42(9):1341-1345.
[15] 张振杰,刘少琼,王波,等.地方品种皖南黄肉种鸡ALV-J与REV的共感染及其分子变异分析[J].中国农业科学,2011,44(11):2379-2386.
[16] 朱美珍,吴玉宝,崔治中.山东地方品系鸡中一株A亚群鸡的白血病病毒的分离和鉴定[J].中国动物传染病学报,2009,17(4):31-35.
[17] 赵冬敏,张青禅,崔治中.芦花鸡中B亚群禽白血病病毒的分离与鉴定[J].病毒学报,2010,26(1):53-57.
[18] 武专昌,朱美珍,边小明,等.两株B亚群禽白血病病毒全基因测序及其在细胞上的复制性比较[J].病毒学报,2011,27(5):447-455.

Preparation and Specific Identification of Single Factor Serum of Subgroup K Avian Leukosis Virus gp85

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WEI Mingbang, BIANBA Qiongda, XIAO Qingqing, DUAN Mengqi, CHAMBA Yangzom, SHANG Peng. Cloning,Bioinformatics and Expression Analysis of CDKN1B Gene in Tibetan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2196-2206.
[2]	XIAO Peng, SHANG Jianghua, YANG Chunyan, LI Mengqi, DUAN Anqin, MA Xiaoya, FENG Chao, HUANG Chenqian, ZHANG Bo, ZHOU Jinchen, WEI Kelong, ZHENG Wei, ZHENG Haiying. Effects of Alpha-linolenic Acid on Buffalo Ovarian Granulosa Cells Cultured in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2380-2387.
[3]	JIAO Linlin, CHENG Yuting, WU Qingguo, WU Shuang, WU Zhi, ZHU Shanyuan, QIAN Yingjuan. Prokaryotic Expression and Polyclonal Antibody Preparation of Duck Tembusu Virus Capsid Protein [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2395-2402.
[4]	LI Can, LI Chuchu, ZENG Wei, ZHANG Ning, JI Chunxiao, CHEN Tao. Recombinant Expression and Function Study of RegⅢγ Protein in Porcine [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2414-2426.
[5]	SONG Yue, SU Shengjie, ZHANG Fan, BAI Fan, DAI Lingli, WANG Na, WANG Dawei, YANG Qianwen, ZHAO Shihua, ZHANG Yuemei. Prokaryotic Expression and Polyclonal Antibody Preparation of Mannheimia haemolytica Truncated Leukotoxin [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2479-2486.
[6]	OU Yunwen, PAN Qin, WANG Yang, DAI Junfei, REN Shaoke, ZHANG Yang, ZHANG Jie. Truncated Expression of ORF2 Gene of Porcine Parvovirus Type 6 and Preparation of Its Polyclonal Antibody [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2487-2495.
[7]	WANG Haorui, YU Baojun, YANG Jiayi, WEI Rong, FU Xi, WANG Chuanchuan, ZHANG Di, LI Desheng, CAI Zhengyun, GU Yaling, ZHANG Juan. Bioinformatics Analysis and Tissue Expression of DERA Gene in Jingyuan Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1764-1773.
[8]	PAN Pengcheng, LEI Zongquan, LI Xian, HU Xiangyun, QIN Qiantao, QIN Zhaoxian, GUAN Zhihui, CHEN Baojian, XIE Bingkun. Bioinformatics Analysis,Eukaryotic Expression Vector Construction and Tissue Expression of MYL2 Gene in Luchuan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1796-1806.
[9]	ZHUO Na, ZHU Zhongwu, LI Jing, HU Shixiang, WANG Jianchang, LIN Hua, CHEN Chaolin, HAN Diangang, AI Jun, CHEN Peifu. Bioinformatics Analysis and Eukaryotic Expression of ORF2 Protein of Swine Hepatitis E Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1959-1970.
[10]	ZHANG Fangyuan, LIN Yating, YANG Dawei, CHEN Hu, LI Guimei, SHAN Hu. Prokaryotic Expression of African Swine Fever Virus P30 Protein and Establishment of Indirect ELISA Detection Method [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2012-2022.
[11]	WANG Xueping, ZHANG Xiaojun, LI Xiaoyue, WANG Guodong, ZHANG Fuliang, SONG Yuwei, ZHANG Mingliang, MA Lei. Prokaryotic Expression of Hexon Protein of Highly Pathogenic Fowl Adenovirus Serotype 4 and Preparation of Polyclonal Antibodies [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2053-2060.
[12]	TAO Qinghua, HUANG Caiyun, CHEN Chao, LIU Wei, SUN Ningning, LI Ang. Cloning and Tissue Expression Difference Analysis of LIF Gene in White Muscovy Duck [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1296-1306.
[13]	LIU Hongrun, ZHU Siran, FENG Lingli, ZHANG Kun, YAN Gang, WANG Yubin, ZHANG Shuai, JIANG Shan, XU Di, LAN Ganqiu, LIANG Jing. Cloning,Bioinformatics Analysis and Tissue Expression Localization of CDO1 Gene in Large White Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1352-1363.
[14]	ZHANG Zhewei, DAI Xiaotong, WEI Zudan, CHEN Yunpeng, GAN Jing, BEI Weicheng. Study on Prokaryotic Expression and Immunogenicity of OmpW and TbpA Proteins in Swine Pasteurella multocida [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1489-1498.
[15]	HU Leyu, HU Xinyao, LI Ying, ZHAO Shengmiao, SHEN Fengzhen, WANG Changfa, LI Liangliang, WANG Tongtong, REN Huiying. Bioinformatics Analysis of HO-1 Gene and Preparation of Polyclonal Antibody in Dezhou Donkey [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1499-1510.