H9N2亚型禽流感病毒HA基因的表达及其单因子血清的制备

doi:10.16431/j.cnki.1671-7236.2015.11.011

Abstract

Abstract: To prepare the mono-specific serum to diagnose H9N2 avian influenza virus (AIV),this test extracted H9N2 subtype AIV RNA and then amplified the upper HA1 gene,the middle HA2 gene and the lower HA3 gene by RT-PCR,respectively.Then they were inserted into expression vector pET-32a(+) and transformed into BL21(Rosetta) expression strain.The expressed proteins were used to immune Kunming White mice to prepare antiserum.Recombinant fusion proteins of HA1 HA2 and HA3 were obtained successfully and they showed good immunogenicity.Indirect immunofluorescence assay (IFA) showed that the two serums obtained by the upper HA1 and the middle HA2 could react with the H9N2 subtype AIV,while that of the lower HA3 could not.Recombinant Marek's disease virus (MDV) MZC12 HA/NA also proved that the serums prepared by HA1 and HA2 could recognize the expression of HA gene.The mono-specific serum of H9N2 subtype AIV was prepared successfully,which could lay the foundation for the diagnosis and research of H9N2 subtype AIV.

Key words: H9N2 subtype avian influenza; HA gene; expression; indirect immunofluorescence assay (IFA); mono-specific serum

CLC Number:

S855.3

CHEN Jun-xia, SUN Peng, XU Shu-zhen, LI Si-fei, SU Shuai, ZHAO Peng, CUI Zhi-zhong. Expression of H9N2 Subtype Avian Influenza Virus HA Gene and Preparation of its Mono-specific Serum[J]. , 2015, 42(11): 2888-2894.

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Expression of H9N2 Subtype Avian Influenza Virus HA Gene and Preparation of its Mono-specific Serum

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