›› 2010, Vol. 37 ›› Issue (10): 63-67.

• 生物技术 • Previous Articles     Next Articles

Primary Studies on the Functions of Differentially Expressed Gene (07E12) in Infective Larvae of Ascaris suum by RNA Interference

HUANG Cui-qin1,2, CHEN Hong-hui1,2, HUANG Qi-chun1,2, CHEN Xing-xing1,2,ZHENG Xin-tian1,2   

  1. (1.College of Life Sciences, Longyan University, Longyan 364000, China;2.Key Laboratory of Preventive Veterinary Medicine and Biotechnology, Longyan University, Longyan 364000, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-10-20 Published:2010-10-20

Abstract: In order to elucidate the function of a key developmental gene using double-stranded RNA mediated interference (RNAi), the EST 07E12 was selected from an Ascaris. suum infective larvae-specific cDNA library. A pair of specific primers was designed to amplify the 07E12 and the dsRNA was synthesized using MEGAscript RNAi kit. The dsRNA was delivered into infective larvae by soaking，and RT-PCR was used to detect the target mRNA after different lengths of soaking time．The results showed that in RNA-treated worms, soaking for 24 h did not result in decreased target mRNA levels; the reduction of 07E12 transcript levels began from 24 to 72 h, and continued to decrease until they were no longer detectable at 96 h．The results indicated that RNAi effect was evident．

Key words: Ascaris suum; infective larvae; RNA interference; function

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