双氢青蒿素对新孢子虫感染雄性小鼠精子质量和生精功能基因的影响

doi:10.16431/j.cnki.1671-7236.2025.04.031

Abstract

Abstract: 【Objective】 Neosporidiosis is a protozoan disease that causes clinical symptoms such as abortion,stillbirth or movement disorder in pregnant animals.The aim of this experiment was to determine the effect of dihydroartemisinin on sperm quality and spermatogenic function genes of male mice infected with Neosporidium neosporidium, in order to acheive anti-Neosporidium neosporidium effect.【Method】 BALB/c male mice model infected with Neosporidium neosporidium were taken as the study object,and dihydroartemisinin was administered intragastically at a dose of 0.2 g/kg for 7 days.Meanwhile,blank control and model groups were set up.After administration,the reproductive organ index and sperm quality of male mice were measured,and the morphological changes of sperm were observed by modified Pap staining.The expression of spermatogenic function genes C-kit,Plzf,Sycp3 and Stra8 were detected at 7th,14th,21st,35th and 42nd days after administration.On the 7th day of administration,the apoptosis of spermatogenic cells was detected by flow cytometry.【Result】 The male mice model of Neosporidium neosporidium infection were established successfully.Compared with blank control group,the reproductive organ index,sperm density and sperm motility of mice in model group were extremely significantly decreased (P＜0.01),while the sperm malformation rate was extremely significantly increased (P＜0.01).The expressions of C-kit,Plzf,Sycp3 and Stra8 genes were significantly or extremely significantly decreased (P＜0.05 or P＜0.01).The apoptosis rate of spermatogenic cells was extremely significantly increased (P＜0.01).In dihydroartemisinin group,the reproductive organ index (excluding the 21st day),sperm density and sperm motility were significantly or extremely significantly decreased (P＜0.05 or P＜0.01),while the sperm malformation rate was significantly increased (P＜0.01).The expression of C-kit,Plzf,Sycp3 and Stra8 genes were decreased,reaching significant levels at some time points (P＜0.05).The apoptosis rate of spermatogenic cells was extremely significantly increased (P＜0.01).Compared with model group,the reproductive organ index of mice in dihydroartemisinin group was significantly or extremely significantly increased (P＜0.05 or P＜0.01),the sperm density and sperm motility were increased,the sperm malformation rate was significantly decreased (P＜0.01).The expression of C-kit,Plzf,Sycp3 and Stra8 genes were increased,reaching significant or extremely significant levels at some time points (P＜0.05 or P＜0.01),and the apoptosis rate of spermatogenic cells was extremely significantly decreased (P＜0.01).【Conclusion】 Dihydroartemisinin could regulate the reproductive organ index of male mice,improve sperm quality,reduce the production of malformed sperm,increase the expression of spermatogenic function genes Sycp3,C-kit,Plzf and Stra8,and improve the damage of Neosporidium neosporidium on spermatogenic sperm quality and spermatogenic function genes of male mice.The results provided a reference for the research of effective anti-Neosporidium neosporidium drugs.

Key words: Neosporidium neosporidium; dihydroartemisinin; reproductive organ index; sperm quality; spermatogenic function genes

CLC Number:

S852.72⁺3

ZHAO Fanglin, LIU Meng, TANG Zeyu, ZHAO Jianhao, LI Lu, JIA Lijun. Effects of Dihydroartemisinin on Sperm Quality and Spermatogenic Function Gene in Male Mice Infected with Neosporidium neosporidium[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(4): 1796-1806.

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Effects of Dihydroartemisinin on Sperm Quality and Spermatogenic Function Gene in Male Mice Infected with Neosporidium neosporidium

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