China Animal Husbandry & Veterinary Medicine ›› 2009, Vol. 36 ›› Issue (9): 79-82.

• 生物技术 • Previous Articles     Next Articles

Rescue of Vero Cell Adapted Rabies Virus ERA Vaccine Strain with R333E Mutation in Glycoprotein

GUO Li1,2, WANG Xi-jun3, FENG Na2, GE Jin-ying3, YANG Song-tao2, XIA Xian-zhu2,BU Zhi-gao3   

  1. (1.Graduate School, Jilin Agriculture University, Changchun 130118, China; 2.The Institute of Veterinary Medicine, Academy of Military Medical Science, Changchun 130000, China; 3.National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-09-20 Published:2009-09-20
  • Contact: XIA Xian-zhu

Abstract: The glycoprotein (G) of rabies virus (RV) is known to play a predominant role in the pathogenesis of rabies. And G333 (Arg) is one of molecular basis that decided neurocyte tropism in rabies virus. For developing new vaccine of rabies virus which is safer and has good immunogenicity, based on reverse genetics, the antigen site R333 of Glycoprotein, in the Vero cell adapted ERA vaccine strain, was mutated from R to E. And rescued the attenuated vaccine strain ERAGΔ successfully. TCID50 was between 10-4 and 10-5, in vitro growth the virus of ERAGΔ was reproduce after 24 hours, and the highest titer was in five days, then fall-off. The result shows the same typical grows kinetics with parents ERA in Vero cells. It was a substantial technique for the next research on attenuated vaccine. In this experiment, the attenuated vaccine strain ERAGΔ was rescued successfully, and provided a good candidate for vaccine. It has great value for next research and practical application.

Key words: rabies virus; reverse genetics; glycoprotein; mutation

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