重金属离子单克隆抗体及免疫检测研究进展

›› 2009, Vol. 36 ›› Issue (9): 67-70.

• 生物技术 • Previous Articles Next Articles

Progress on Monoclonal Antibodies Specific for Heavy Metal Ion-chelator Complexes and Immunoassays to Detect Heavy Metal Ions

KONG Tao, LI Xiao-bing, LIU Guo-wen, XIE Guang-hong, ZHANG Zhi-gang, ZHANG Yi, XU Chao, WANG Zhao-peng, WANG Zhe

(Animal Science and Veterinary Medicine College of Jilin University, Changchun 130062, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-09-20 Published:2009-09-20
Contact: WANG Zhe

Abstract

Abstract: Immunoassays for heavy metal ions are new methods to detect heavy metal ions. Compared to traditional methods, immunoassays are not only fast, cheap, simple, but also reasonably portable. And they will permit the real-time/on-site assessment of heavy metal contamination. There are two prototype assays polyclonal antibody immunoassay and monoclonal antibody immunoassay, including fluorescence polarization immunoassay, enzyme linked immunosorbent assay and immunosensor assay. Immunoassays based on preparation of monoclonal antibody specific for various heavy metal ion-chelator complexes may have great potential for practical applications. 

Key words: heavy metals; monoclonal antibody; immunoassay

CLC Number:

Q813.2

KONG Tao;LI Xiao-bing;LIU Guo-wen;XIE Guang-hong;ZHANG Zhi-gang;ZHANG Yi;XU Chao;WANG Zhao-peng;WANG Zhe . Progress on Monoclonal Antibodies Specific for Heavy Metal Ion-chelator Complexes and Immunoassays to Detect Heavy Metal Ions[J]. , 2009, 36(9): 67-70.

[1]	HOU Haoyu, ZHENG Nannan, WU Hongju, CHEN Yinlong, LI Chao, ZHANG Angke, JI Pengchao, WAN Bo, DU Yongkun, ZHANG Gaiping. Preparation and Characterization of Monoclonal Antibody Against p30 Protein of African Swine Fever Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1241-1249.
[2]	WANG Leming, WANG Yurui, ZENG Zixuan, RAO Guoshun, WU Zhengjiao, JIN Weikun, WANG Dongying. Preparation of Cathepsin L1 Monoclonal Antibody Against Fasclala gigantica and Establishment of Double Antibody Sandwich ELISA [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 677-686.
[3]	MO Hongfang, JIE Kai, CHEN Xin, WEN Wei, LIU Wenqiang, CHEN Huanchun, QIAN Ping, LI Xiangmin. Preparation and Identification of Monoclonal Antibodies Against Porcine Seneca Valley Virus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 307-317.
[4]	HU Huanyi, WANG Jianzhong, LIU Changjin, LIN Min, LIU Xiaolan, WEI Huangsiwu, DENG Shunzhou. Eukaryotic Expression of Green Fluorescent Protein and Preparation of Its Monoclonal Antibody [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 328-337.
[5]	ZENG Jianyu, DONG Zhenyuan, JIA Wenfeng, ZHANG Guozhong, XUE Jia. Preparation and Identification of Monoclonal Antibody Against Newcastle Disease Virus M Protein [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3423-3431.
[6]	LI Si, JIANG Wei, CHENG Tingting, LU Chunhui, XIN Sipei, CAO Jingzheng, WANG Quan, SUN Weidong. Establishment of an Indirect Competitive Chemiluminescence Enzyme-linked Immunoassay for Sarafloxacin [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1772-1783.
[7]	YANG Songxin, FENG Jianyuan, GUO Xuan, WEI Liuyuan, LIANG Yiying, YAN Hao, ZHANG Ziyi, CHEN Hailan. Preparation of Monoclonal Antibodies for Small Molecular Compounds and Their Applications in the Field of Veterinary Medicine [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 1121-1131.
[8]	LI Jiesen, SUN Ronghang, KUANG Yanqi, CHEN Luman, LIU Qing, GUO Xiaofeng. Optimal Expression of Porcine Epidemic Diarrhea Virus S1D Protein and Preparation of Its Monoclonal Antibody [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4641-4651.
[9]	YIN Yongkang, WANG Xiaoliang, CUI Yanli, LIANG Xiao. Research Progress on the Detection and Analysis Methods of Nicarbazine in Poultry Products and Feed [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4725-4734.
[10]	WANG Zhiping, ZHANG Yunjing, SUN Yujie, XU Xin, WANG Zhiyan, HUANG Baicheng, TIAN Kegong. Development and Evaluation of an Indirect ELISA for Detection of Porcine Epidemic Diarrhea Virus IgA in Pig Serum and Milk Captured by Monoclonal Antibody [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 2248-2255.
[11]	SHI Jianzhou, LI Qingmei, WANG Yanhong, LIU Xiao, LI Ge, GUO Junqing, DENG Ruiguang, ZHANG Gaiping. Establishment of a Method of IPMA for Detection of the Monoclonal Antibody Against H1N1 Swine Influenza Virus [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(9): 2691-2698.
[12]	XIANG Zilai, WANG Ling, XIA Sugan, ZOU Hui, GU Jianhong, YUAN Yan, LIU Xuezhong, LIU Zongping, BIAN Jianchun. Preparation of Monoclonal Antibodies Against Phenylethanolamine A and Establishment of ELISA Method [J]. , 2019, 46(3): 849-857.
[13]	ZHOU Manli, ZHOU Yucheng, ZHANG Haiwei, QIAO Lianjiang, YANG Yanling. Preparation and Detection of Monoclonal Antibodies Against Mycobacterium bovis MPB70 Protein [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(10): 3075-3083.
[14]	FAN Suju, YANG Xingdong, GAO Run. Preparation of Chloramphenicol Monoclonal Antibody and Establishment of ELISA Detection Method [J]. , 2018, 45(9): 2566-2574.
[15]	WANG Lingling, SUN Guopeng, XU Ruonan, HE Haixun, YAN Zhanpeng, WANG Yaping, XING Ruilin, REN Pengju, LI Peng, ZHANG Yanfang, ZHU Yanping, YUE Feng, WANG Xuannian. Study on Preparation and Biological Function of Chicken PD-1 Monoclonal Antibody [J]. , 2018, 45(12): 3363-3370.

Progress on Monoclonal Antibodies Specific for Heavy Metal Ion-chelator Complexes and Immunoassays to Detect Heavy Metal Ions

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments