扩展莫尼茨绦虫引发酶蛋白基因的克隆和cDNA序列分析

›› 2009, Vol. 36 ›› Issue (9): 59-62.

• 生物技术 • Previous Articles Next Articles

Cloning and Sequence Analysis of the cDNA Encoding Primase in Moniezia expansa

LV Ting-de^1，2, ZHAO Wen-juan², ZHU Jian-jun², WANG Xin-hua², BO Xin-wen²

(1.Shihezi University, Shihezi 832000, China; 2.The Breed and Biotechnology Key Laboratory of Sheep in Xinjiang Bingtuan, Shihezi 832000, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-09-20 Published:2009-09-20
Contact: BO Xin-wen

Abstract

Abstract: To clone and identify novel genes from an adult Moniezia expansa (M.expansa) cDNA library, and provide a foundation for further research, a cDNA library was constructed from M.expansa adult stage. Clones were selected randomly from the cDNA library and were sequenced by using the method of expression sequence tags (ESTs). Novel genes were acquired by primer-walking. The cDNA sequence encoding M.expansa primase protein was analyzed, including searching the ORF, translating the nucleotide to protein sequence, similarity searches and secondary structure predication with bioinformatics analysis. Primase genes, 1269 bp and coding for 422 amino acids, was cloned and sequenced, then the sequence was submitted to GenBank and got an accession number,GH291475. The theoretical pI was 4.83 and molecular weight was 47.1598 ku. The full-length cDNA sequence encoding M.expansa primase was obtained, which gave a basis for further functional study of this gene.

Key words: M.expansa; primase; sequence analysis; ESTs

CLC Number:

LV Ting-de;;ZHAO Wen-juan;ZHU Jian-jun;WANG Xin-hua;BO Xin-wen. Cloning and Sequence Analysis of the cDNA Encoding Primase in Moniezia expansa[J]. , 2009, 36(9): 59-62.

[1]	SONG Chun, WANG Bolin, LI Mei, WEN Ming, WANG Kaigong, CHEN Changxiu, CHENG Zhentao. Isolation and Identification of Mycoplasma synoviae Guizhou Strain and Sequence Analysis of VlhA Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1516-1523.
[2]	WEI Yunfang, LI Feixiang, Xing Yun, Huang Qingguo, LI Judong, WAN Jiusheng, CHEN Fangliang. Study on Cloning, Bioinformatics Analysis and Spatio-temporal Expression of IGF2 Gene in Kunming Dogs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 866-875.
[3]	HE Xianming, REN Guangcai, YE Junxian, LAN Hong, LIU Yufu, XIONG Ting, YANG Zekun, XU Ting, CHEN Ruiai. Isolation and Identification of a Strain of Infectious Bursal Disease Virus and Sequence Analysis of VP2 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1005-1014.
[4]	CAI Jie, WU Jiayi, PAN Yuanxia, HU Gaowei, ZHANG Linzhou. Isolation, Identification and Drug Resistance Analysis of Chryseobacterium sp. from Pseudosciaena crocea [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1135-1143.
[5]	HU Xiaobing, LIN Biaosheng, WANG Zhenwei, ZHENG Lijing. Isolation, Identification and Genomic Sequence Analysis of a Strain of Clostridium butyricum from Pigs Utilizing Lactic Acid [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 569-578.
[6]	KOU Meiling, MIAO Haisheng, LI Le, XIE Jiarui, LI Huachun, SONG Jianling. Isolation and Identification of Chuzan Virus from Palyam Serogroup Virus in Yunnan Border Area [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4401-4409.
[7]	TAN Bin, WANG Chao, WANG Yan, ZHANG Shuqin. Isolation and Identification of Parvovirus from Palm Civets and Sequence Analysis of VP2 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 4012-4018.
[8]	WANG Zhiyan, SUN Yujie, XU Xin, HUANG Baicheng, TIAN Kegong. Isolation,Identification and Pathogenicity of Porcine Deltacoronavirus Strain HeN10 [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2982-2989.
[9]	LI Nan, HE Yuwen, ZUO Yuanyuan, MENG Jinxin, XU Tiangang, WANG Jinglin. Analysis of Molecular Biological Characteristics of Structural Genes of Getah Virus (SZC30) Isolated from Midge [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1149-1160.
[10]	ZHANG Qi, CHEN Haotian, SHAN Jiabao, CHANG Rui, ZHANG Xue, WANG Yao, WANG Junwei, MA Bo. Cloning and Sequence Analysis of NS1 and VP1 Genes of Gosling Plague Virus from Jilin District [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1161-1169.
[11]	GUO Mengjiao, LI Mingtao, TAN Huihui, ZHANG Chengcheng, ZHANG Xiaorong, WU Yantao. Study on Cloning and Expression Characteristic of NLRC3 Gene in Rabbit [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 3940-3949.
[12]	WANG Zhengfan, ZHU Lisai, LI Fei, XIANG Rui, LI Yiyun, YING Biyun, WANG Guiping, BAI Aiquan, JIA Aiqing. Pathogenicity and Whole Genome Sequence Analysis of Porcine Deltacoronavirus CH7328 Strain [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 4143-4153.
[13]	YIN Dejing, ZHANG Yundan, YUE Jun, LI Qiaobin, HE Ling, LI Tao, WANG Kaigong, CHENG Zhentao. Whole Genomic Sequencing Analysis of Fowl Adenovirus Serotype 4 from Guizhou [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(1): 44-54.
[14]	PAN Pengcheng, WEN Binhua, XIE Wan, JIANG Changjin, JIAO Di, CHEN Baojian, GUAN Zhihui, XIE Bingkun. Sequence Analysis,Eukaryotic Expression Vector Construction and Tissue Expression Analysis of PDK4 Gene in Luchuan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2337-2347.
[15]	QIN Benyuan, YANG Shuai, ZHANG Yanwei, LI Wenxin, LIU Hong, WU Yiqi, ZHANG Xuelian, LI Wenxia, YANG Yang, CAI Chunbo, GAO Pengfei, GUO Xiaohong, LI Bugao, CAO Guoqing. Study on Cloning,Bioinformatics Analysis and Expression Profile of ASB2 Gene CDS in Pig [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1289-1298.

Cloning and Sequence Analysis of the cDNA Encoding Primase in Moniezia expansa

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments