Abstract: pathogens collected in the laboratory were used for isolation and culture, staining, microscopy, animal test, and biochemical test; disk diffusion method was used to screen sensitive drugs, aluminum aluminum adjuvant was sgnthetized in the laboratory, own inactivated vaccine was prepared to control their disease. The experimental results showed that, the is olate strain was Haemophilus parasuis, isolated strains were sensitive to Cefotaxime, Amikacin, Gentamicin and Cephalosporins; own inactivated vaccine to control the disease was safe and effective. 

Key words: Haemophilus parasuis; isolation and identification; own inactivated vaccine