›› 2009, Vol. 36 ›› Issue (6): 90-94.

• 遗传繁育 • Previous Articles     Next Articles

Single Nucleotide Polymorphism Analysis of IFN-γ Gene in Pigs

WANG Da-li1，2，LIU Chang1，WANG Xiu-na2，HUANG Da-peng2，SUN Bo-xing1，ZHAO Zhi-hui1   

  1. (1.College of Animal Science and Veterinary Medicine of Jilin University, Changchun 130062,China; 2.College of Animal Science and Technology, Heilongjiang August First Land Reclamation University, Daqing 163319, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-06-20 Published:2009-06-20
  • Contact: SUN Bo-xing

Abstract: Interferon-gamma (IFN-γ) plays an important role in the innate host defense against viral infection. In order to analyze the value that the IFN-γ gene act as a genetic marker in porcine disease resistance, single nucleotide polymorphisms (SNPs) in the whole region of the IFN-γ gene were studied by use of PCR-SSCP approach in 481 pigs from the “Junmu No. 1” White, Landrace, Duroc pigs. Three SNP sites were detected: T825C in Intron 1, T2730C in Intron 3 and G5301A in Extron 4. Except for the absence of G5301A in Duroc pigs, all the other sites in the all populations were in a state of Hardy-Weinberg equlibrium.The PIC of every sites had low polymorphisms(<0.25), which represented the own unique conservativeness of IFN-γ gene in pigs.

Key words: pig; IFN-γ; single nucleotide polymorphisms (SNP)

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