拟态弧菌OmpK基因的克隆及原核表达

China Animal Husbandry & Veterinary Medicine ›› 2009, Vol. 36 ›› Issue (6): 52-55.

• 生物技术 • Previous Articles Next Articles

Cloning and Prokaryotic Expression of OmpK Gene of Vibrio mimicus

LI Yan-dong¹，LI Fei²， LU Shi-ying¹，REN Hong-lin¹，ZHOU Yu¹， ZHU Yuan-yin³， GONG Bin-bin¹，LIU Zeng-shan¹

(1.Key Laboratory for Zoonosis Research, Ministry of Education, Institute of Zoonosis,College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China; 2.Hebei Feed Industry Association, Shijiazhuang 050031, China; 3.Animal Quarantine Station of Ningxia Zhongwei, Zhongwei 755000, China)

Received:1900-01-01 Revised:2009-03-24 Online:2009-06-20 Published:2009-06-20

Abstract

Abstract: A target of common antigen, OmpK (outer membrane protein K), is the receptor for KVP40 which is abroad-host-rang Vibriophage. To investigate the possibility of OmpK as species antigen, primers were designed according to OmpK gene sequence published in GenBank, and a piece of DNA sequence 801 bp was amplified by PCR from genomic DNA of Vibrio mimicus. The OmpK gene of Vibrio mimicus was cloned with 801 nucleotides encoding 267 amino acids. The OmpK gene was expressed in Escherichia coli induced by IPTG. The molecular weight of recombinant protein was about 28 ku.

Key words: Vibrio mimicus; OmpK gene; clone; expression

CLC Number:

LI Yan-dong;LI Fei;LU Shi-ying;REN Hong-lin;ZHOU Yu;ZHU Yuan-yin;GONG Bin-bin;LIU Zeng-shan. Cloning and Prokaryotic Expression of OmpK Gene of Vibrio mimicus[J]. China Animal Husbandry & Veterinary Medicine, 2009, 36(6): 52-55.

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Cloning and Prokaryotic Expression of OmpK Gene of Vibrio mimicus

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