不同胎牛血清对动物细胞体外培养的影响

›› 2009, Vol. 36 ›› Issue (4): 96-99.

• 遗传繁育 • Previous Articles Next Articles

Effect of Different Types of Serum on the Cultured in Vitro of Animal Fibroblasts

WU Hong-mei1, LIU Shuai1,2, BAO A-dong1,2, LU Tao-feng1, LIU Chang-qing1,3， ZHANG Wen-xiu1, 
GUAN Wei-jun1, MA Yue-hui1

(1.Institute of Animal Science，Chinese Academy of Agricultural Sciences， Beijing 100193，China； 2.College of Animal Science and Medicine，Agricultural University of Inner Mongolia，Hohhot 010018，China;3.Division of Life Science and Technology，Ocean University of China，Qingdao 266003，China)

Received:1900-01-01 Revised:2008-11-26 Online:2009-04-20 Published:2009-04-20
Contact: GUAN Wei-jun

Abstract

Abstract: In order to study the basic effect of different serum on the construction of cell line, fibroblast cells were isolated from 20 seven-day old embryo of short-feet chicken and cultured, passaged and frozen via the method of adhere culture using 2 kind of FBS. The cell number, flask number, morphology and cell growth curve were observed. The results showed that various serum had significant effect on cell culture, and the cell characteristics were better when cultured with serum B than that with serum A. The present study provide the reference to the choice of serum on cell culture.

Key words: serum; animal fibroblasts; in vitro culture

CLC Number:

Q813.1⁺1

WU Hong-mei;LIU Shuai;;BAO A-dong;;LU Tao-feng;LIU Chang-qing;;ZHANG Wen-xiu;GUAN Wei-jun;MA Yue-hui. Effect of Different Types of Serum on the Cultured in Vitro of Animal Fibroblasts [J]. , 2009, 36(4): 96-99.

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Effect of Different Types of Serum on the Cultured in Vitro of Animal Fibroblasts

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