鸳鸯鸭MHC-Ⅰ α链基因的克隆与分子进化分析

›› 2009, Vol. 36 ›› Issue (12): 60-62.

• 生物技术 • Previous Articles Next Articles

Cloning and Sequence Analysis of Yuanyang Duck MHC-I αChain Mature Peptide Gene of the Extracellular Domain

SUN Kai^1,2, LI Xin-sheng^1,2, CUI Bao-an^1,2, CHEN Hong-ying^1,2, WEI Zhan-yong^1，2, ZHANG Lei^1,2, SONG Ya-peng^1,2, RUAN Wu-ying^1,2

(1.The College of Animal Husbandry and Veterinary, Henan Agricultural University, Zhengzhou 450002, China;2.Henan Animal Food Safety Key Laboratory, Zhengzhou 450002, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-12-20 Published:2009-12-20
Contact: CUI Bao-an

Abstract

Abstract: Yuanyang duck MHC class Iα chain cDNA was completely amplified from a duck spleen by RT-PCR, then cloned and sequenced. The result showed cloned duck MHC class Iα chain gene span is 909 bp, which encodes 303 amino acid residues, including a complete Yuanyang duck MHC-Iα chain mature peptide (813 bp) of the extracellular domain. The results of homology analysis with other duck MHC-Iα chain mature peptide gene of the extracellular domain revealed that there was identity of 89.4% to 91.0% at nucleotide homology. This study paved the way for future study of expression, biological function and application of duck MHC-Iα chain mature peptide of the extracellular domain.

Key words: Yuanyang duck; MHC-1α chain mature peptide gene; cloning; sequencing

CLC Number:

SUN Kai;LI Xin-sheng;CUI Bao-an;CHEN Hong-ying;WEI Zhan-yong;ZHANG Lei;SONG Ya-peng;RUAN Wu-ying;. Cloning and Sequence Analysis of Yuanyang Duck MHC-I αChain Mature Peptide Gene of the Extracellular Domain[J]. , 2009, 36(12): 60-62.

[1]	CHEN Kaiwen, ZHAO Yunjiao, WANG Lei, ZHANG Jianbo, MA Yuhong, WU Guofang. Transcriptome Analysis of Porcine Leydig Cells Under Different Oxygen Concentrations [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2185-2195.
[2]	SHI Jingu, HUANG Shanshan, HUANG Jiwei, CHEN Kunhai, HU Dasheng, ZHU Zhixiong, HAN Shuyu. Comparative Transcriptome Analysis of Streptococcus agalactiae from Tilapia Under High Temperature Stress [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2207-2216.
[3]	PIAN Huifang, DU Xubin, LI Yan, ZHANG Yuchen, LIU Fei, YU Debing. Sequencing Analysis of the Full-length Transcriptome of Granulosa Cells of Small Yellow Follicle in Hy-Line Variety Brown [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1754-1763.
[4]	FAN Bingfeng, LI Wen, LIU Lixiang, ZHAO Xiangyuan, SHAO Jing, LIN Lefeng, SUN Huimin, ZHANG Xulin, XU Baozeng. Cloning and Bioinformatics Analysis of AANAT Gene in Mink [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1307-1318.
[5]	LIU Hongrun, ZHU Siran, FENG Lingli, ZHANG Kun, YAN Gang, WANG Yubin, ZHANG Shuai, JIANG Shan, XU Di, LAN Ganqiu, LIANG Jing. Cloning,Bioinformatics Analysis and Tissue Expression Localization of CDO1 Gene in Large White Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1352-1363.
[6]	MA Jifu, BAKHET·Bodai, KEZERBEK·Kazibek, LI Zhenwei, ORALHAZI·Hazikhan. Screening and Identification of Inhibin α Subunit-specific Nanobody Gene [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1532-1542.
[7]	LI Cuihan, QU Zhina, GAO Yubin, LI Yan, WANG Juan, DUAN Xiaoxiao, WANG Lin, ZHANG Xiyue, ZHAO Ge, HUANG Xiumei, ZHAO Jianmei, ZHANG Qingqing, WANG Junwei, HUANG Baoxu, LIU Junhui. Epidemiology Investigation and Whole Genome Sequencing Analysis of Three Types of Poultry Originated Carbapenem-resistant Escherichia coli [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1653-1662.
[8]	ZHANG Yongjie, SUI Zhiyuan, ZHANG Zhishuai, WANG Chenguang, LI Xiaojun, XING Feng. Cloning and Bioinformatics Analysis of NPTX1 Gene and Its Tissues Expression at Puberty in Dolang Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1048-1058.
[9]	LIU Kexin, WANG Chao, ZHANG Ao, LIU Jiali, TAN Bin, ZHANG Shuqin. Isolation,Identification and Genome Sequence Analysis of Bovine Viral Diarrhea Virus LN-1 Strain [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1150-1159.
[10]	WEI Yirong, ZHENG Zihua, ZHANG Sanbao, SONG Ying, JIANG Haiyu, SUN Wenyue, CHENG Pengfei, LIU Yufan, ZOU Jianwei, HUANG Yanna, PAN Yan, JIANG Qinyang. Cloning,Bioinformatics Analysis and Tissue Expression of UCP1 Gene in Nubian Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 440-450.
[11]	ZHANG Jiupan, MA Qing, WANG Jin, MA Lina, WEI Dawei, LIANG Xiaojun. Single-cell RNA Sequencing Technology and Its Application in the Study of Intramuscular Adipocytes [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 479-489.
[12]	SONG Peijia, JIN Yipeng, LIN Degui, LIN Jiahao. Clinical Research Progress on Canine Periodontal Diseases [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 838-845.
[13]	CHEN Yanxi, WANG Chen, LUO Yuan, ZHOU Yuancheng, XU Zhiwen, PENG Yuanyi, SONG Zhenhui, LIU Xiao. circRNA Expression Profile Analysis of PK-15 Cells Response to Seneca Virus A Infection [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 280-289.
[14]	TANG Jingyu, DU Hanyu, MENG Chunchun, LIU Guangqing. Cloning,Bioinformatics Analysis and Subcellular Localization of Interferon-stimulated Gene 15 in Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2843-2854.
[15]	QI Nannan, QIAN Yonghang, LUO Gang, SITU Xuqi, LIU Jiying. Cloning, Bioinformatics Analysis and Tissue Expression Location of Porcine MORC2 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2431-2441.

Cloning and Sequence Analysis of Yuanyang Duck MHC-I αChain Mature Peptide Gene of the Extracellular Domain

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments