China Animal Husbandry & Veterinary Medicine ›› 2009, Vol. 36 ›› Issue (12): 60-62.

• 生物技术 • Previous Articles     Next Articles

Cloning and Sequence Analysis of Yuanyang Duck MHC-I αChain Mature Peptide Gene of the Extracellular Domain

SUN Kai1,2, LI Xin-sheng1,2, CUI Bao-an1,2, CHEN Hong-ying1,2, WEI Zhan-yong1，2, ZHANG Lei1,2, SONG Ya-peng1,2, RUAN Wu-ying1,2   

  1. (1.The College of Animal Husbandry and Veterinary, Henan Agricultural University, Zhengzhou 450002, China;2.Henan Animal Food Safety Key Laboratory, Zhengzhou 450002, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-12-20 Published:2009-12-20
  • Contact: CUI Bao-an

Abstract: Yuanyang duck MHC class Iα chain cDNA was completely amplified from a duck spleen by RT-PCR, then cloned and sequenced. The result showed cloned duck MHC class Iα chain gene span is 909 bp, which encodes 303 amino acid residues, including a complete Yuanyang duck MHC-Iα chain mature peptide (813 bp) of the extracellular domain. The results of homology analysis with other duck MHC-Iα chain mature peptide gene of the extracellular domain revealed that there was identity of 89.4% to 91.0% at nucleotide homology. This study paved the way for future study of expression, biological function and application of duck MHC-Iα chain mature peptide of the extracellular domain.

Key words: Yuanyang duck; MHC-1α chain mature peptide gene; cloning; sequencing

CLC Number: