籽鹅促卵泡激素α亚基基因的克隆、序列分析及其原核表达载体的构建

›› 2009, Vol. 36 ›› Issue (11): 77-81.

• 生物技术 • Previous Articles Next Articles

Cloning, Sequence Analysis and Construction of Prokaryotic Expression Vector of the FSH Alpha Subunit Gene in Zi Goose

GUO Jing-ru¹, YANG Huan-min¹, CAI Jun¹, LI Peng¹, KANG Bo²

(1.College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 2.College of Animal Husbandry and Veterinary Medicine, Jilin University, Changchun 130062, China）

Received:1900-01-01 Revised:1900-01-01 Online:2009-11-20 Published:2009-11-20
Contact: YANG Huan-min

Abstract

Abstract: The total RNA was extracted from the pituitary of Zi goose, and the cDNA of the FSHα was amplified by RT-PCR. The amplified FSHα gene is 396 bp in length. The FSHα gene was cloned into the vector pMD18-T. The positive clones were verified and sequenced. The nucleotide sequence and deduced amino acid sequence were compared with homologous sequences from available species including chicken, quail, turkey, mouse, sheep, cattle, et al. Sequence alignment showed that FSHα gene in these species were highly conservative. FSHα gene had the highest homology of 95.9% with chicken, quail and turkey in nucleotide, and had the highest homology of 97.5% with chicken, quail and turkey in amino acids. Then the clones of FSHα gene were inserted into pET-32a (+) prokaryotic expression vector.

Key words: Zi goose; follicle stimulating hormone alpha subunit; cloning

CLC Number:

Q785

GUO Jing-ru;YANG Huan-min;CAI Jun;LI Peng;KANG Bo. Cloning, Sequence Analysis and Construction of Prokaryotic Expression Vector of the FSH Alpha Subunit Gene in Zi Goose[J]. , 2009, 36(11): 77-81.

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Cloning, Sequence Analysis and Construction of Prokaryotic Expression Vector of the FSH Alpha Subunit Gene in Zi Goose

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