籽鹅促卵泡激素β亚基cDNA的分子克隆与序列分析

Abstract

Abstract: The total RNA obtained from the pituitary of Zi goose was used to amplify the cDNA for the precursor of Zi Goose follicle stimulating hormone β subunit by RT-PCR, and the opening reading frame of 396 bp was acquired. The fragments amplified were ligated with pMD18-T vector and transformed into DH5α competent cells. The positive clones were verified, sequenced, and submitted to GenBank, with accession No. EU563910. The nucleotide sequence and deduced amino acid sequence were compared with homologous sequences from available animals including sheep, buffalo, chicken, duck, tiger, and human. Sequence alignment showed a fairly high degree of homology, duck being ranked the highest. As a whole, various animals exist the homology of follicle stimulating hormone β subunit.

Key words: Zi goose; follicle stimulating hormone; β subunit; cloning; sequence analysis

CLC Number:

Q785

CAI Jun;YANG Huan-min;LI Peng;GUO Li. Cloning and DNA Sequence Analysis of the cDNA for the Precursor of Zi Goose Follicle Stimulating Hormone β Subunit[J]. China Animal Husbandry & Veterinary Medicine, 2009, 36(10): 47-49.

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Cloning and DNA Sequence Analysis of the cDNA for the Precursor of Zi Goose Follicle Stimulating Hormone β Subunit

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