›› 2008, Vol. 1 ›› Issue (7): 85-86.

• 疾病防治 • Previous Articles     Next Articles

Homeomorphic Cultivation Research of Chicken Newcastle Disease Virus and Infectious Bronchitis Virus

XUE Zhang-lin, ZHANG Shu-bin, LIU Rui-sheng, GOU Xiang-zhen, WANG Bi-hui   

  1. Instiute of Gansu Veterinary and Animal Science, Pingliang 744000,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-07-20 Published:2008-07-20

Abstract: Chicken Newcastle disease virus(NDV) and infectious bronchitis virus(IBV)were diluted according to a certain proportion, then weve mixed equally, and the mixtures of the two virus were inoculated to 9~10 dog-old chicken embryos to research the proliferation of them in identical chicken embryos (abbreviating as homeomorphic cultivation). The results showed that: Inoculation of ND-Lasota strain (1∶5) and IBH120 strain (1∶5000) mixture, by allantoic cavity didnit influenced proliferation of the two virus in the identical chicken embryos. Hemagglutination titers of the two virus (NDV and IBV ) obtained after 96 h were respectively 11 log2 and 12 log2 by hemagglutination test, which were not lower than that of each one. Virus proliferation was tend to balance. Meanwhile, replication of NDV and IBV couldn’t produce interference effect each other under proper condition cultivation.

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