Abstract: Using PCR technology, the lactoferricin gene was obtained from genome of Gaoyuan yak; The Lfcin gene was cloned to pGEM-T easy vector, and the product was sequenced by biocompany; Gaoyuan yak and cow’s Lfcin sequences were aligned; Yak, cow, human, mouse’s Lfcin protein sequences were analysed. As a result, the DNA sequence that contains the LF gene second exon of Gaoyuan yak was cloned, constituted by 778 bp, Lfcin coding region that code 25 amino acids being 75 bp length; According sequence analyse, there are 9 different bases between cow and yak’s sequence; Yak and cow’s Lfcin gene code same protein sequence and there are high homology in different species.

Key words: yak; Lfcin gene; clone