蓝狐与银狐褪黑激素受体1A基因的克隆及序列分析

Abstract

Abstract: A pair of primers were designed to amplify the sequences of melatonin receptor 1A from arctic fox and silver fox according to the sequence homology of MTNR1A from other animal species. PCR product was separated, purified and cloned with the pUCm-T vector. The cloned sequences were 546 bp in length(GenBank Accession No. EU170442 and EU170443). The homology of the nucleotide sequence and amino acid sequence was 99.1% and 99.4% between arctic fox and silver fox, respectively. They had 84% to 86% homology to MTNR1A sequences of human, mouse and sheep, and 74% to MTNR1A sequence of chicken.

Key words: arctic fox; silver fox; melatonin receptor 1A; sequence analysis

CLC Number:

Q785

YAN Shou-qing;WANG Hui-fang;ZHU Wan-ju  . Cloning and Sequence Analysis of Melatonin Receptor 1A from Arctic Fox and Silver Fox[J]. China Animal Husbandry & Veterinary Medicine, 2008, 1(3): 24-26.

[1]	LI Tianxiu, LI Xinpeng, DONG Xinxing, LAN Guoxiang, YAN Dawei, ZHU Jiawei. Amplification,Sequence Analysis and Tissue Expression Study of HMOX2 Gene in Lijiang Pigs [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(5): 2219-2231.
[2]	ZHANG Xuedi, XU Kai, YIN Shuo, LIU Jing, WANG Zhonghao, QIN Jianhua, WU Pengyuan, WANG Chuanwen. Cloning,Sequence Analysis and Expression Characteristics of Carboxylesterase 2 Gene in Dermanyssus gallinae [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(7): 2973-2983.
[3]	HUANG Liqing, DUAN Anqin, ZHENG Haiying, YANG Chunyan, SHANG Jianghua. Sequence Analysis,Eukaryotic Expression Vector Construction and Tissue Expression of SFRP1 Gene in Buffalo [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(5): 1807-1818.
[4]	HAN Hui, GUO Yajing, YAN Guangzhi, CHEN Shengnan, LIU Mingjie, MO Meilian, HUANG Liangzong. Isolation,Identification and Genetic Evolution Analysis of a Eurasian Avian-like Swine Influenza Virus [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(4): 1642-1650.
[5]	BIJunming, DONG Yaqin, CUIJin, SHA Zhou, GU Congcong, ZHENG Hui, WEI Rong, SUN Fuliang, ZHANG Feng, ZHANG Zhihong. Isolation，Identification and Genetic Evolution Analysis of a Strain of Bovine Viral Diarrhea Virus in Shandong Province [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(10): 4485-4499.
[6]	WU Aoqi, LUO Yuhang, REN Tongwei, WANG Hao, DONG Qinting, QIN Yifeng, WEI Zuzhang, OUYANG Kang, CHEN Ying, HUANG Weijian, PAN Yan, LI Fengmei, XIE Jiang. Isolation，Identification and Genetic Evolution Analysis of a Strain of Bovine Parvovirus Type 1 in Guangxi [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(10): 4540-4549.
[7]	DOU Junfeng, WANG Zui, LI Li, LU Qin, JIN Xinxin, LING Xiaochun, LUO Qingping, ZHAI Xinguo. Identification of Subgroups A, B and J Avian Leukosis Virus Co-infection and Sequence Analysis of gp85 Gene [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(1): 302-311.
[8]	KANG Danju, PENG Qiuyun, ZHOU Jiaxin, CHEN Jinjun, LIN Hongying, ZHOU Guangxian. Cloning, Bioinformatics Analysis and Tissue Expression of AvBD1 and AvBD2 Genes in Yellow-feather Chickens [J]. China Animal Husbandry & Veterinary Medicine, 2023, 50(11): 4325-4337.
[9]	XING Tianqi, XU Di, YAN Gang, WANG Yubin, WANG Mengying, LIANG Jing, LAN Ganqiu. Sequence Analysis, Eukaryotic Expression Vector Construction and Tissue Expression of ABCD4 Gene in Bama Miniature Pigs [J]. China Animal Husbandry & Veterinary Medicine, 2023, 50(11): 4338-4348.
[10]	LI Nan, MENG Jinxin, WANG Jiannie, HE Yuwen, YANG Shaochang, LYU Shunyan, WANG Jinglin. Detection of Lumpy Skin Disease Virus in Cattle and Investigation of Virus Carried by Midges and Mosquitoes [J]. China Animal Husbandry & Veterinary Medicine, 2023, 50(10): 4179-4187.
[11]	LIU Huayun, ZHANG Lei, XU Guili, ZHANG Tianhao, XIE Yuchun, DUAN Lingxin, LIU Zhengzhu, GONG Yuanfang. Identification of the Core Promoter Region of MC1R Gene in Silver Fox [J]. China Animal Husbandry & Veterinary Medicine, 2022, 49(7): 2442-2450.
[12]	SONG Chun, WANG Bolin, LI Mei, WEN Ming, WANG Kaigong, CHEN Changxiu, CHENG Zhentao. Isolation and Identification of Mycoplasma synoviae Guizhou Strain and Sequence Analysis of VlhA Gene [J]. China Animal Husbandry & Veterinary Medicine, 2022, 49(4): 1516-1523.
[13]	WEI Yunfang, LI Feixiang, Xing Yun, Huang Qingguo, LI Judong, WAN Jiusheng, CHEN Fangliang. Study on Cloning, Bioinformatics Analysis and Spatio-temporal Expression of IGF2 Gene in Kunming Dogs [J]. China Animal Husbandry & Veterinary Medicine, 2022, 49(3): 866-875.
[14]	HE Xianming, REN Guangcai, YE Junxian, LAN Hong, LIU Yufu, XIONG Ting, YANG Zekun, XU Ting, CHEN Ruiai. Isolation and Identification of a Strain of Infectious Bursal Disease Virus and Sequence Analysis of VP2 Gene [J]. China Animal Husbandry & Veterinary Medicine, 2022, 49(3): 1005-1014.
[15]	CAI Jie, WU Jiayi, PAN Yuanxia, HU Gaowei, ZHANG Linzhou. Isolation, Identification and Drug Resistance Analysis of Chryseobacterium sp. from Pseudosciaena crocea [J]. China Animal Husbandry & Veterinary Medicine, 2022, 49(3): 1135-1143.

Cloning and Sequence Analysis of Melatonin Receptor 1A from Arctic Fox and Silver Fox

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments