Abstract: In order to establish pathological model of chickens’ infectious laryngotracheitis by artificial infection with simulating natural methods. 80 days old Jingfen chickens were randomly divided into 1 blank control group and 3 experimental groups with 10-4,10-5,10-6 dosages, 20 chickens per group in this paper. The chickens’ infectious laryngotracheitis virus(ILTV) was droped into chickens’ larynx and trachea through mouth. To be observed the clinical results and statistic the incidence of the disease and the mortality. The results indicate that the 10-4group’s delitescence was 1～8 days,incidence of the disease was 100%, death rate was 10%； the 10-5group’s delitescence was 2～8 days,incidence of the disease was 100%, death rate was 5%； the 10-6group’s delitescence was 4～9 days,incidence of the disease was 90%, death rate was 0%. Per experimental group all can show the same clinical symptoms and pathologic symptom. But the 10-5group is more typical and accord ing with natural infection. So the 10-5group can be used as the artificial pathologic model.

Key words: infectious laryngotracheitis; artificial infection; infection dosage; infection route