不同方法检测鸡白痢沙门氏菌抗体消长规律的比较研究

doi:10.16431/j.cnki.1671-7236.2018.02.026

Abstract

Abstract:

To compare and evaluate the different methods for detection Salmonella Pullorum (S. Pullorum) antibody, and to provide scientific guidance for the purification of breeding chicken farms, SPF chickens were inoculated with active and inactivated S. Pullorum. Serum samples were collected and detected by three different methods (rapid serum agglutination, micro-agglutination and ELISA), and Kappa test was applied to determine the concordance among different methods. The results showed that positive samples were detected earlier when using rapid serum agglutination than ELISA, but the later method had a longer detection period above the threshold on positive samples, which was more consistent with the dynamic rule of antibody in S. Pullorum positive chickens. It showed weak agreement among three different methods (Kappa value were 0.002 to 0.295). In conclusion, the study demonstrated that there were big differences among three methods for S. Pullorum antibody detection. ELISA might be the best choice in single S. Pullorum infection, because of its no false positive interference, longer detection period above the threshold on S. Pullorum positive samples and more consistent with the dynamic rule of antibody.

Key words: Salmonella Pullorum; artificial infection; antibody; rapid serum agglutination test; micro-agglutination test; ELISA; Kappa test

CLC Number:

S858.31

LIU Yang, LI Dan, WANG Chuanbin, HUO Siqi, LIU Yuliang, GU Xiaoxue. A Comparative Study of Various Methods for the Detection of Dynamic Rule of the Antibody Against Salmonella Pullorum[J]. , 2018, 45(2): 493-500.

References

[1] 李瑞香,张亚娟,薛九利,等. 鸡白痢的检测及综合净化措施[J]. 黑龙江畜牧兽医,2016,2:117-118.LI R X, ZHANG Y J, XUE J L, et al. Detection and comprehensive purification of Salmonella Pullo-rum[J]. Heilongjiang Animal Science and Veterinary Medicine,2016, 2:117-118. (in Chinese)
[2] 张璐,张雪伟,袁宗辉,等. 畜禽沙门氏菌病防制研究进展[J]. 中国畜牧兽医,2017,44(7):2103-2111.ZHANG L, ZHANG X W, YUAN Z H, et al. Research progress on salmonellosis prevention and control in livestock and poulty[J]. China Animal Husbandry & Veterinary Medicine,2017,44(7):2103-2111. (in Chinese)
[3] 施开创,李凤梅,邹联斌,等. 鸡源致病性沙门氏菌的分离鉴定及血清型和药物敏感性分析[J]. 中国畜牧兽医,2015,42(8):2160-2168.SHI K C, LI F M, ZOU L B, et al. Isolation, identification and analysis of serotype and antibiotic susceptibility of pathogenic Salmonella from chickens[J]. China Animal Husbandry & Veterinary Medicine,2015,42(8):2160-2168. (in Chinese)
[4] PARVEJ M S, NAZIR K H, RAHMAN M B, et al. Prevalence and characterization of multi-drug resistant Salmonella enterica serovar Gallinarum biovar Pullorum and Gallinarum from chicken[J]. Veterinary World,2016,9(1):65-70.
[5] GONG J, ZHANG J, XU M, et al. Prevalence and fimbrial genotype distribution of poultry Salmonella isolates in China (2006 to 2012)[J]. Applied and Environmental Microbiology,2014,80(2):687-693.
[6] GONG J, XU M, ZHU C, et al. Antimicrobial resistance, presence of integrons and biofilm formation of Salmonella Pullorum isolates from Eastern China (1962-2010)[J]. Avian Pathology, 2013, 42(3):290-294.
[7] 黄秀英,陈晓娇,张妹. 蛋种鸡场鸡白痢检测方法与净化程序[J]. 中国畜牧杂志,2012,48(2):71-72.HUANG X Y, CHEN X J, ZHANG M. Detection method and purification procedure of Salmonella Pullorum in laying breeding farms[J]. Chinese Journal of Animal Science,2012, 48(2):71-72. (in Chinese)
[8] 王飞,冯泽清,刘益平,等. 二郎山山地鸡不同品系的鸡白痢净化效果分析[J]. 中国畜牧兽医,2014,41(5):216-220.WANG F, FENG Z Q, LIU Y P, et al. Purifying effect analysis of Pullorum between lines in Erlang Mountain chickens[J]. China Animal Husbandry & Veterinary Medicine,2014,41(5):216-220. (in Chinese)
[9] 李海琴,魏岳,刘林秀,等. 东乡黑鸡禽白血病及鸡白痢净化效果研究[J]. 中国兽医杂志,2016,52(7):85-86.LI H Q, WEI Y, LIU L X, et al. Study on the avian leukosis and Salmonella Pullorum purification effect of black chicken in Dongxiang[J]. Chinese Journal of Veterinary Medicine,2016,52(7):85-86. (in Chinese)
[10] DAILEY N, NIEMEIER D, ELKHORAIBI C, et al. Descriptive survey and Salmonella surveillance of pastured poultry layer farms in California[J]. Poult Science,2017,96(4):957-965.
[11] 费维斌. 无为县蛋鸡场鸡白痢血清流行病学调查[J]. 当代畜禽养殖业,2017,5:56.FEI W B. Epidemiological survey of Salmonella Pullorum in laying poultry farms of Wuwei county[J]. Modern Animal Husbandry,2017,5:56. (in Chinese)
[12] 孙向东,刘拥军,蔡丽娟,等. 美国家禽改良计划特点和管理结构[J]. 中国动物检疫,2009,26(7):64-66.SUN X D, LIU Y J, CAI L J, et al. Characteristics and management structure of national poultry improvement program[J]. China Animal Health Inspection,2009,26(7):64-66. (in Chinese)
[13] 刘洋,顾小雪,杨卫铮,等. 种鸡场鸡白痢净化效果及可行性分析[J]. 中国家禽,2017,39(11):71-73.LIU Y, GU X X, YANG W Z, et al. Purification effect and feasibility of Salmonella Pullorum in breeding farms[J]. China Poultry,2017,39(11):71-73. (in Chinese)
[14] 于丽萍,王永玲,陈向前,等. 家禽和生猪健康促进策略研究[J]. 中国动物检疫,2014,2:10-13.YU L P, WANG Y L, XU X Q, et al. Research on poultry and swine health promotion strategy[J]. China Animal Health Inspection,2014,2:10-13. (in Chinese)
[15] 丁进,曹钟鑫,丁丹,等. 郧阳大鸡白痢净化效果观测[J]. 养殖与饲料,2016,4:77-78.DING J, CAO Z X, DING D, et al. Purification effect on Salmonella Pullorum of Yunyang chicken[J]. Animals Breeding and Feed,2016,4:77-78. (in Chinese)
[16] 王海源,徐家芳,莫国东,等. 种鸡场鸡白痢、鸡伤寒净化抗原比较及检测方法的确定[J]. 中国家禽,2014,36(2):52-53.WANG H Y, XU J F, MO G D, et al.Comparison and determination of Salmonella Pullorum and Salmonella Gallinarum antibody detection reagent in breeding farms[J]. China Poultry,2014,36(2):52-53. (in Chinese)
[17] 刘军,刘家彦,宋克磊,等. 人工感染鸡白痢菌抗体消长规律的试验研究[J]. 畜牧兽医科技信息,2006,5:16-17.LIU J, LIU J Y, SONG K L, et al. A study of dynamic rule on Artificial infection of Salmonella Pullorum antibody[J]. Graziery Veterinary Sciences,2006,5:16-17. (in Chinese)
[18] 耿士忠,刘欢,焦新安,等. 鸡白痢沙门菌SpiC蛋白的原核表达及其应用[J]. 细胞与分子免疫学杂志,2015,31(2):149-152.GENG S Z, LIU H, JIAO X A, et al. Prokaryotic expression and application of SpiC protein of Salmonella Pullorum[J]. Chinese Journal of Cellular and Molecular Immunology,2015,31(2):149-152. (in Chinese)
[19] 郑世军,徐志超,李晓齐,等. 一种检测鸡白痢沙门氏菌抗体的ELISA试剂盒[P].2015.10.14.ZHENG S J, XU Z C, LI X Q, et al. An ELISA kit for the detection of Salmonella Pullorum antibo-dies[P].2015.10.14. (in Chinese)
[20] GUO R, LI Z, JIAO Y, et al. O-polysaccharide is important for Salmonella Pullorum survival in egg albumen, and virulence and colonization in chicken embryos[J]. Avian Pathol,2017,46(5):535-540.
[21] 徐耀辉,焦新安,胡青海,等. 酶标单抗阻断ELISA检测鸡白痢和鸡伤寒抗体[J]. 中国兽医学报,2006,26(2):140-143.XU Y H, JIAO X A, HU Q H, et al. A blocking ELISA for the diagnosis of Pullorum disease and fowl typhoid by using peroxidase-labelled monoclonal antibody and Salmonella Pullorum lipopolysaccha-ride[J]. Chinese Journal of Veterinary Science, 2006,26(2):140-143. (in Chinese)
[22] OIE. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. Fowl Typhoid and Pullorum Disease[Z]. 2012.

A Comparative Study of Various Methods for the Detection of Dynamic Rule of the Antibody Against Salmonella Pullorum

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	JIAO Linlin, CHENG Yuting, WU Qingguo, WU Shuang, WU Zhi, ZHU Shanyuan, QIAN Yingjuan. Prokaryotic Expression and Polyclonal Antibody Preparation of Duck Tembusu Virus Capsid Protein [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2395-2402.
[2]	OU Yunwen, PAN Qin, WANG Yang, DAI Junfei, REN Shaoke, ZHANG Yang, ZHANG Jie. Truncated Expression of ORF2 Gene of Porcine Parvovirus Type 6 and Preparation of Its Polyclonal Antibody [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2487-2495.
[3]	ZHANG Fangyuan, LIN Yating, YANG Dawei, CHEN Hu, LI Guimei, SHAN Hu. Prokaryotic Expression of African Swine Fever Virus P30 Protein and Establishment of Indirect ELISA Detection Method [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2012-2022.
[4]	WANG Xueping, ZHANG Xiaojun, LI Xiaoyue, WANG Guodong, ZHANG Fuliang, SONG Yuwei, ZHANG Mingliang, MA Lei. Prokaryotic Expression of Hexon Protein of Highly Pathogenic Fowl Adenovirus Serotype 4 and Preparation of Polyclonal Antibodies [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2053-2060.
[5]	HU Leyu, HU Xinyao, LI Ying, ZHAO Shengmiao, SHEN Fengzhen, WANG Changfa, LI Liangliang, WANG Tongtong, REN Huiying. Bioinformatics Analysis of HO-1 Gene and Preparation of Polyclonal Antibody in Dezhou Donkey [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1499-1510.
[6]	WANG Wenjie, RU Penghui, YU Chuan, DU Fuxi, CHEN Songbiao, SHANG Ke, ZHANG Chunjie, CHENG Xiangchao. Prokaryotic Expression of VP2 Protein of Luoyang Feline Panleukopenia Virus Isolate and Polyclonal Antibody Preparation [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1511-1521.
[7]	ZHANG Guoqing, JIANG Yuhang, ZHANG Shuang, LI Letian, HAO Jiayi, CHEN Jing, ZOU Wancheng, LI Chang, LI Taiyuan, GAO Xu, JIN Ningyi. Preparation and Identification of PDCoV RBD Protein Peptide Antibody [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1575-1583.
[8]	HE Lin, HAN Diangang, LI Jing, DU Xuan, ZHANG Jiaxiang, XIN Jige. The Progress of the Detection Methods of Bovine Viral Diarrhea Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1593-1602.
[9]	MU Yijiao, YU Ruiming, ZHANG Liping, WANG Yonglu. Establishment and Application of ELISA for Detection of IgA Antibody Based on the Recombinant Full-length S2 Protein of Porcine Epidemic Diarrhea Virus Variant Strain [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1185-1194.
[10]	NIU Xiaojie, XU Mingguo, XIAO Li, LIU Wenhao, CHEN Chuangfu, WANG Yong. Expression and Purification of IPAJ Protein of Salmonella Pullorum, Preparation of Polyclonal Antibody and Establishment of ELISA Method [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1218-1228.
[11]	HOU Haoyu, ZHENG Nannan, WU Hongju, CHEN Yinlong, LI Chao, ZHANG Angke, JI Pengchao, WAN Bo, DU Yongkun, ZHANG Gaiping. Preparation and Characterization of Monoclonal Antibody Against p30 Protein of African Swine Fever Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1241-1249.
[12]	XIAO Li, NIU Xiaojie, LIU Qingqing, WANG Yueli, CHEN Chuangfu, YI Jihai. Construction of Recombinant Adenovirus with VP4 Gene of Porcine Rotavirus and Evaluation of Antibody Level [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 260-269.
[13]	ZHANG Kai, ZHANG Chunping, GE Shengqiang, SUN Chunxi, CHENG Jie, FU Chunyu, WANG Gang, NIU Xing, PENG Jun. Expression of p22 Protein of African Swine Fever Virus,Preparation of Polyclonal Antibody and Establishment of Indirect ELISA Method [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 270-279.
[14]	ZHANG Mengyang, HE Jian, LIU Yangkun, YAO Lunguang. Bioinformatics Analysis and Polyclonal Antibody Preparation of African Swine Fever Virus p37 Protein [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 300-309.
[15]	ZHU Lilin, WANG Houkun, CHEN Xueyang, LIU Jing, LIANG Xiongyan, FANG Chun, YANG Yuying. Effect of Chicken IFIT5 on Replication of J Subgroup Avian Leukemia Virus Within DF-1 Cells [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 310-316.