Abstract: There are abundant of preantral follicles in mammalian ovary. To study preantral follicles,there is very important significance on oogenesis and follicular development.And make the best of ovary resources to promote animal breeding,preserve endangered species and human reproduction health.There have been significant scientific advances since researching porcine preantral follicles.The aim of this paper is to present a comprehensive review of recent achievements on porcine preantral follicles in vitro culture methods ,culture condition, existence problem and development perspective.

Key words: porcine; preantral follicles; in vitro culture